Gene | PM (μg/ml) | LPS (μg/ml) |
---|
| 1 | 5 | 10 | 0.1 | 0.5 | 1.0 |
---|
COX-2 | 10.6 ± 0.2a
| 15.9 ± 1.4a
| 43.5 ± 2.4a
| 14.5 ± 1.3a
| 48.2 ± 2.4a
| 82.1 ± 4.1a
|
TNF-α | 8.1 ± 0.3a
| 19.8 ± 0.6a
| 42.5 ± 1.2a
| 9.4 ± 1.4a
| 16.8 ± 1.4a
| 42.2 ± 2.6a
|
IL-6 | 3.2 ± 0.4a
| 7.5 ± 0.4a
| 22.1 ± 0.7a
| 6.2 ± 0.4a
| 14.2 ± 1.0a
| 25.2 ± 1.1a
|
IL-8 | 9.3 ± 0.4a
| 17.4 ± 2.1a
| 35.6 ± 1.7a
| 12.8 ± 1.4a
| 22.5 ± 1.3a
| 51.6 ± 1.3a
|
SAA1 | 1.6 ± 0.4 | 2.7 ± 0.4a
| 4.6 ± 0.3a
| 2.0 ± 0.2a
| 3.2 ± 0.3a
| 7.8 ± 1.2a
|
- U937 macrophages were treated with 1.0, 5.0, or 10.0 μg/ml PM C (size cutoff: 2.1 μm) from dairy farm #57 for 6 h. As a positive control, cells were treated with 0.1, 0.5, or 1.0 μg/ml LPS for 6 hours and mRNA was analyzed by real time RT-PCR. Control cells received 1% PBS. Results are normalized to β-actin and given as fold increase of the mRNA levels in treated cells versus controls (= 1). Values are given as mean ± SD of triplicates of three independent experiments
-
asignificantly increased compared to control cells (p < 0.05)