Lymphoblasts were treated with NaAsO
at 0, 10, 20, 50 and 100 μM for two, four or eight hours. Levels of DNA methylation expressed as % of controls were illustrated as a dose-response and time-course study on global LINE-1 methylation determined by COBRA and quantified by capillary gel electrophoresis using a Bioanalyzer (A), dose-response of arsenite treatment on total 5-MedC content determined by HPLC-MS/MS in lymphoblasts at four hours of treatment (B) and p53 promoter methylation in lymphoblasts treated with arsenite at 50 μM for four hours (C). Each point represents the mean ± SE from three independent experiments. *,** and *** represent statistically significant difference from control at p < 0.05, 0.01 and p < 0.001, respectively.