Lymphoblasts were treated with NaAsO
(0.0, 0.5 and 1.0 μM) for two up to eight weeks. The experiment was performed twice. Dose-response and time-course of arsenite treatment on levels of DNA methylation expressed as % of controls was determined as Global LINE-1 methylation determined by COBRA, quantified by capillary gel electrophoresis using a Bioanalyzer (A), total 5-MedC content determined by HPLC-MS/MS (B) and p53 promoter methylation (C). Each point represents the mean ± SE from three independent experiments. * and ** represent statistically significant difference from control at p < 0.05 and p < 0.01, respectively.