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Table 1 Primers and location of CpG sites that were quantified by pyrosequencing

From: A panel study of occupational exposure to fine particulate matter and changes in DNA methylation over a single workday and years worked in boilermaker welders

Sequence ID

Forward Primer

Reverse Primer

Sequencing Primer

Sequence analyzed

 

(5′ to 3′)

(5′ to 3′)

(5′ to 3′)

 

Global methylation analysis

 Alu

Biotin-TTTTTATTAAAAATATAAAAATT

CCCAAACTAAAATACAATAA

AATAACTAAAATTACAAAC

G/A C/TG/A C/TG/ A CCACCA

 LINE-1

TTTTGAGTTAGGTGTGGGATATA

Biotin-AAAATCAAAAAATTCCCTTTC

AGTTAGGTGTGGGATATAGT

TTC/T GTGGTGC/T GTC/T G

Gene-specific methylation analysis

 iNOS

TTGGATGGTATGGGGTGAGTAT

Biotin-TACCCAATCCCCTCATCAA

GTGTGTTTATAATTTTGTAG

C/T GAGTC/T GAAAATTGAGGTTTC/T GG

  1. Nucleotides where DNA methylation was measured are in bold text.