Figure 2
From: Pooling samples for “top-down” molecular exposomics research: the methodology
When the random grouping numbers of pools risen to five, the frequency of non-significant difference between the case and control data with log-normal distributions reached down to the value of that the difference calculated by individual data*. The pool numbers (i.e., numbers of pools in x-axis) was set as 2, 3, 4, and 5 and coded as G2, G3, G4, and G5; 100 times random grouping was applied to the 200 artificial data with log-normal distributions for the case and control populations, respectively; the means of the case are 1.1, 1.6, 2.3, 4.9 and 6.8-folds of the control; the means of the case are 1.1, 1.6, 2.3, 4.9 and 6.8-folds of the control and coded as D5, D4, D3, D2 and D1, respectively. *For the upper mentioned log-normal distribution data, calculated differences by using five random pool means of the case and control, respectively, should be close to by using the individual data.