Figure 3

Effects of test samples on pathological changes in the lungs. (A) Control, (B) Tar 1: no pathologic alterations were seen in the lungs. (C) Tar 5: very slight infiltration of inflammatory cells in the airway submucosa. Goblet cells were not seen. (D) OVA alone: slight proliferation of goblet cells in the airway epithelium and very slight infiltration of inflammatory cells into the airway submucosa. (E) OVA + Tar 1: goblet cell proliferation was somewhat stronger than in the samples treated with OVA alone (D). (F) OVA + Tar 5: mild proliferation of goblet cells in the airway epithelium, and infiltration of inflammatory cells into the airway submucosa. (G) H-ASD + OVA: mild goblet cell proliferation and mild infiltration of inflammatory cells into the airway submucosa. (H) H-ASD + OVA + Tar 1: moderate goblet cell proliferation, severe infiltration of inflammatory cells into the airway submucosa. (I) H-ASD + OVA + Tar 5: mild to moderate goblet cell proliferation, moderate infiltration of inflammatory cells into the airway submucosa. (A–I) PAS stain; bar = 50 μm.