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Table 2 Retention time and estrogenic response of indicative compounds

From: Assessment of xenoestrogenic exposure by a biomarker approach: application of the E-Screen bioassay to determine estrogenic response of serum extracts

Compound Rt MOEC RPP
   μM RPE ± SD  
Endogenous compounds
Cholesterol 1.83 0.0001–10 Nonactive -
5α-dihydroprogesterone 4.14 10 58 ± 3 0.01
Progesterone 5.03 25 16 ± 2 0.004
Pregnenolone 6.07 10 92 ± 6 0.01
Androsterone 6.21 10 97 ± 3 0.01
5α-dihydrotestosterone 6.63 1 64 ± 5 0.1
Androstenedione 6.80 10 16 ± 2 0.01
Dehydroepiandrosterone 6.86 1 92 ± 2 0.1
Testosterone 8.23 10 34 ± 5 0.01
Estrone 9.06 0.01 84 ± 8 10
17α-hydroxyprogesterone 9.23 10 7 ± 1 0.01
17α-hydroxypregnenolone 12.78 10 30 ± 1 0.01
17β-estradiol 14.83 0.001 100 ± 7 100
16α-hydroxyestrone 22.86 0.01 77 ± 2 10
Estriol 24.01 0.01 103 ± 3 10
Organochlorines
Hexachlorobenzene 1.34 0.001 – 50 Nonactive -
PCB180 1.40 0.001 – 50 Nonactive -
p,p'-DDE 1.43 12.5 92 ± 2 0.008
PCB153 1.48 0.001 – 50 Nonactive -
PCB138 1.49 0.001 – 50 Nonactive -
o,p'-DDT 1.53 6.25 96 ± 7 0.016
Dieldrin 1.75 25 44 ± 9 0.004
Methoxychlor 2.29 + 2.88 12.5 57 ± 8 0.008
4-OH-CB146 2.67 0.001 – 50 Nonactive -
4-OH-CB187 2.67 50 24 ± 4 0.002
4-OH-CB107 2.70 0.001 – 50 Nonactive -
β-hexachlorocyclohexane 4.13 2.5 61 ± 2 0.04
  1. Abbreviations: Rt, retention time; MOEC, maximum observed effect concentration; RPE, relative proliferative effect; RPP, relative proliferative potency