Gene
|
N
|
Mean Fold Changea ± SE
|
P-valueb
|
---|
IL-11
|
4
|
1.3 ± 0.2
|
0.27
|
IL-7
|
5
|
0.9 ± 0.2
|
0.58
|
GM-CSF
|
3
|
Increasedc
|
-
|
G-CSF
|
4
|
Increasedc
|
-
|
LIF
|
5
|
5.7 ± 1.2
|
0.02*
|
IL-6
|
7
|
7.0 ± 1.5
|
0.01*
|
SCF
|
4
|
1.1 ± 0.1
|
0.40
|
M-CSF
|
5
|
1.0 ± 0.1
|
0.79
|
TGF-β1
|
2
|
1.2, 1.4
|
0.26
|
TGF-β3
|
2
|
0.8, 0.9
|
0.18
|
MIF
|
2
|
0.9, 1.0
|
0.52
|
-
a From the Ribonuclease Protection Assays (RPAs), mean fold-change was determined by normalizing the optical density band of the LPS-treated sample to the housekeeping gene GAPDH or L-32, then expressing it relative to the optical density of the normalized band from the vehicle-treated sample. b Based on one-sample t-test to determine whether mean was significantly different from maximum LPS induction (μ = 1.0). c Expression of GM-CSF and G-CSF was sometimes undetectable in unstimulated cells, thus the LPS-induced expression could not be described appropriately as "fold change" * p < 0.05