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Table 1 LPS induces GM-SCF, G-CSF, LIF, and IL-6 mRNAs in bone marrow stromal cells

From: Aryl hydrocarbon receptor (AhR) agonists suppress interleukin-6 expression by bone marrow stromal cells: an immunotoxicology study

Gene N Mean Fold Changea ± SE P-valueb
IL-11 4 1.3 ± 0.2 0.27
IL-7 5 0.9 ± 0.2 0.58
GM-CSF 3 Increasedc -
G-CSF 4 Increasedc -
LIF 5 5.7 ± 1.2 0.02*
IL-6 7 7.0 ± 1.5 0.01*
SCF 4 1.1 ± 0.1 0.40
M-CSF 5 1.0 ± 0.1 0.79
TGF-β1 2 1.2, 1.4 0.26
TGF-β3 2 0.8, 0.9 0.18
MIF 2 0.9, 1.0 0.52
  1. a From the Ribonuclease Protection Assays (RPAs), mean fold-change was determined by normalizing the optical density band of the LPS-treated sample to the housekeeping gene GAPDH or L-32, then expressing it relative to the optical density of the normalized band from the vehicle-treated sample. b Based on one-sample t-test to determine whether mean was significantly different from maximum LPS induction (μ = 1.0). c Expression of GM-CSF and G-CSF was sometimes undetectable in unstimulated cells, thus the LPS-induced expression could not be described appropriately as "fold change" * p < 0.05