Aryl hydrocarbon receptor (AhR) agonists suppress interleukin-6 expression by bone marrow stromal cells: an immunotoxicology study

Table 1 LPS induces GM-SCF, G-CSF, LIF, and IL-6 mRNAs in bone marrow stromal cells

Gene	N	Mean Fold Change^a ± SE	P-value^b
IL-11	4	1.3 ± 0.2	0.27
IL-7	5	0.9 ± 0.2	0.58
GM-CSF	3	Increased^c	-
G-CSF	4	Increased^c	-
LIF	5	5.7 ± 1.2	0.02*
IL-6	7	7.0 ± 1.5	0.01*
SCF	4	1.1 ± 0.1	0.40
M-CSF	5	1.0 ± 0.1	0.79
TGF-β1	2	1.2, 1.4	0.26
TGF-β3	2	0.8, 0.9	0.18
MIF	2	0.9, 1.0	0.52

^a From the Ribonuclease Protection Assays (RPAs), mean fold-change was determined by normalizing the optical density band of the LPS-treated sample to the housekeeping gene GAPDH or L-32, then expressing it relative to the optical density of the normalized band from the vehicle-treated sample. ^b Based on one-sample t-test to determine whether mean was significantly different from maximum LPS induction (μ = 1.0). ^c Expression of GM-CSF and G-CSF was sometimes undetectable in unstimulated cells, thus the LPS-induced expression could not be described appropriately as "fold change" * p < 0.05

ISSN: 1476-069X