Figure 2

Transcriptional activation of exogenous AhR in the N2a-Rα cells. (A) AhR mRNA in N2a-Rα was detected by RT-PCR using primers specific for AhR. In N2a-Vc and Neuro2a, the primers amplified no products. The left panel shows that the primers amplified both mouse and rat AhR. Representative data are presented from triplicate experiments. (B) AhR protein (96kDa) in N2a-Rα was detected by Western blot analysis using a monoclonal antibody specific for AhR. In N2a-Vc and Neuro2a cells, no signals were detected. Representative data are presented from triplicate experiments. (C) The expression of CYP1A1, AhRR and GST mRNAs was detected in N2a-Rα cells. Since these genes were XRE-mediated genes, expression of these mRNAs are functional markers of AhR activation. Representative data are presented from triplicate experiments.