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Figure 3 | Environmental Health

Figure 3

From: Over-expression of AhR (aryl hydrocarbon receptor) induces neural differentiation of Neuro2a cells: neurotoxicology study

Figure 3

Suppression of CYP1A1 mRNA expression by si-AhR. These graphs demonstrate the repression of AhR and CYP1A1 mRNA expression by si-AhR transfection. N2a-Rα cells were transfected with AhR-targeted siRNA (si-AhR) or scramble siRNA. RNA was isolated from the cells transfected with siRNA and the expression levels were quantified by real-time quantitative PCR. AhR mRNA expression was effectively repressed by si-AhR (panel "AhR"). CYP1A1 mRNA expression was also decreased in cells transfected with si-AhR (panel "CYP1A1"). Scramble siRNA did not exhibit any effect on the mRNA expressions of AhR and CYP1A1 compared with Mock. The results are normalized to the expression level of cyclophilin mRNA and expressed relative to the value in the Mock. Values are presented as the mean of ± S. D. of four replicate determinations; ** p < 0.01 and *p < 0.05.

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