Table 2 Primer sequences used for the amplification of cytokine and housekeeping gene transcripts in lymphocytes
From: Mercury immune toxicity in harbour seals: links to in vitro toxicity
Gene | Primer sequence (5'-3') | Direction | Annealing temperature | Nucleotide position | Calculated length of amplicon | Sequences used for primer pair selection (accession number, NCBI) |
|---|---|---|---|---|---|---|
IL-2 | TTT AAG TTT TAC ACG CCC AAG TGT TTC AGA TCC CTT TAG TTTC | S AS | 55°C | 218–400 | 183 pb | AF072871 |
IL-4 | ACT CAC CAG CAC CTT TGT CCA TCC TTA TCG CTT GTT CTT TG | S AS | 49°C | 48–200 | 153 pb | AF187322, AF104245 |
TGF-β | TTC CTG CTC CTC ATG GCC AC GCA GGA GCG CAC GAT CAT GT | S AS | 57°C | 826–845 1218–1199 | 393 pb | [51] |
GAPDH | GCC AAA AGG GTC ATC ATC TC GGG GCC ATC CAC AGT CTT CT | S AS | 57°C | 1225–1244 1452–1433 | 228 pb | [51] |