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Table 1 Distribution of Haptoglobin (Hp), MnSOD, CAT, GPx-1, ACE, GSTM1 and GSTT1 allele frequencies, genetic diversity parameters, genotype frequencies and Hardy-Weinberg equilibrium data for chi-square (χ2) test.

From: Gene polymorphisms against DNA damage induced by hydrogen peroxide in leukocytes of healthy humans through comet assay: a quasi-experimental study

Genetic Markers Allele frequencies Heterozygosity-observed (Ho) Heterozygosity-expected (He) FIS
(Inbreeding coefficient)
Genotypes Genotype frequencies Number of observed individuals Number of expected individuals HWE test
(p-values)
Hp Hp*1F 0.144 0.570 0.594 0.046 1F-1F 0.04 6 2.75 0.132
       1F-1S 0.06 8 13.05  
  Hp*1S 0.333     1S-1S 0.12 16 14.89  
       1F-2 0.14 19 20.44  
  Hp*2 0.522     1S-2 0.37 50 47.17  
       2-2 0.27 36 36.69  
MnSOD*    0.704 0.498 -0.409 Val/Val 0.18 24 37.74 < 0.001
  Val 0.530     Val/Ala 0.70 95 67.51  
  Ala 0.470     Ala/Ala 0.12 16 29.74  
CAT*    0.385 0.488 0.214 AA 0.23 31 23.94 0.012
  A 0.422     AT 0.39 52 66.11  
  T 0.578     TT 0.39 52 44.94  
GPx-1    0.407 0.432 0.059 Pro/Pro 0.48 65 63.27 0.551
  Pro 0.685     Pro/Leu 0.41 55 58.46  
  Leu 0.315     Leu/Leu 0.11 15 13.27  
ACE    0.467 0.482 0.034 DD 0.36 49 47.88 0.721
  D 0.596     ID 0.47 63 65.24  
  I 0.404     II 0.17 23 21.88  
GSTM1* - 0.622     Null 0.62 84 52.23 < 0.001
  - - -        
  + 0.378     Non-null 0.38 51 82.77  
GSTT1* - 0.244     Null 0.24 33 8.04 < 0.001
  - - -        
  + 0.756     Non-null 0.76 102 126.96  
  1. *p < 0.05 indicates deviation from Hardy-Weinberg equilibrium, appropriate to a heterozygote deficit for CAT locus (p = 0.0085) and a heterozygote excess for MnSOD locus (p = 0.0000). For co-dominant markers (Hp, MnsOD, CAT, GPX-1 and ACE) p-values were generated using statistical programme Genepopweb version 4.0 http://genepop.curtin.edu.au; for dominant markers (GSTM1 and GSTT1), they were calculated by a chi-square calculator programme. For GSTM1 and GSTT1 genotypes were considered: Null = -/-; Non-null = +/+ and +/-.