Gene polymorphisms against DNA damage induced by hydrogen peroxide in leukocytes of healthy humans through comet assay: a quasi-experimental study

Table 1 Distribution of Haptoglobin (Hp), MnSOD, CAT, GPx-1, ACE, GSTM1 and GSTT1 allele frequencies, genetic diversity parameters, genotype frequencies and Hardy-Weinberg equilibrium data for chi-square (χ²) test.

Genetic Markers	Allele frequencies		Heterozygosity-observed (H_o)	Heterozygosity-expected (H_e)	F_IS (Inbreeding coefficient)	Genotypes	Genotype frequencies	Number of observed individuals	Number of expected individuals	HWE test (p-values)
Hp	Hp*^1F	0.144	0.570	0.594	0.046	1F-1F	0.04	6	2.75	0.132
						1F-1S	0.06	8	13.05
	Hp*^1S	0.333				1S-1S	0.12	16	14.89
						1F-2	0.14	19	20.44
	Hp*²	0.522				1S-2	0.37	50	47.17
						2-2	0.27	36	36.69
MnSOD*			0.704	0.498	-0.409	Val/Val	0.18	24	37.74	< 0.001
	Val	0.530				Val/Ala	0.70	95	67.51
	Ala	0.470				Ala/Ala	0.12	16	29.74
CAT*			0.385	0.488	0.214	AA	0.23	31	23.94	0.012
	A	0.422				AT	0.39	52	66.11
	T	0.578				TT	0.39	52	44.94
GPx-1			0.407	0.432	0.059	Pro/Pro	0.48	65	63.27	0.551
	Pro	0.685				Pro/Leu	0.41	55	58.46
	Leu	0.315				Leu/Leu	0.11	15	13.27
ACE			0.467	0.482	0.034	DD	0.36	49	47.88	0.721
	D	0.596				ID	0.47	63	65.24
	I	0.404				II	0.17	23	21.88
GSTM1*	-	0.622				Null	0.62	84	52.23	< 0.001
	-		-	-
	+	0.378				Non-null	0.38	51	82.77
GSTT1*	-	0.244				Null	0.24	33	8.04	< 0.001
	-		-	-
	+	0.756				Non-null	0.76	102	126.96

*p < 0.05 indicates deviation from Hardy-Weinberg equilibrium, appropriate to a heterozygote deficit for CAT locus (p = 0.0085) and a heterozygote excess for MnSOD locus (p = 0.0000). For co-dominant markers (Hp, MnsOD, CAT, GPX-1 and ACE) p-values were generated using statistical programme Genepopweb version 4.0 http://genepop.curtin.edu.au; for dominant markers (GSTM1 and GSTT1), they were calculated by a chi-square calculator programme. For GSTM1 and GSTT1 genotypes were considered: Null = -/-; Non-null = +/+ and +/-.

ISSN: 1476-069X