Table 1 Sequences of the specific primers used in Real-time PCR
Gene | RefSeq accession | Sequence 5’ to 3’ | Amplicon lenght | |
|---|---|---|---|---|
GAPDH | NM_008084.2 | fw | ACCCAGAAGACTGTGGATGG | 172 bp |
rev | ACACATTGGGGGTAGGAACA | |||
ACTB | NM_007393.3 | fw | TTGCTGACAGGATGCAGAAG | 238 bp |
rev | GAAAGGGTGTAAAACGCAGC | |||
OXT (Neurophysin I) | NM_011025.4 | fw | CCCCAGTCTCGCTTGCTGCC | 191 bp |
rev | CGAAGCAGCCCAGCTCGTCC | |||
AVP (Neurophysin II) | NM_009732.1 | fw | AGCCCGAGTGCCACGACGGT | 146 bp |
rev | GGGCTTGGCAGAATCCACGGAC | |||
OXTR | NM_001081147.1 | fw | ACCATCCTGCTCGCCTGGGT | 197 bp |
rev | ATGCCCTCTGGGGCTCTCCG | |||
AVPR1a | NM_016847.2 | fw | CCCTTGCCTCGGACAAGCCG | 226 bp |
rev | GTGGGAGCCTCGCGGGAAAC | |||
ESR1 (ERα) | NM_007956.4 | fw | CTCAACCGCCCGCAGCTCAA | 223 bp |
rev | CCCCCAGGCTGTTGGCACTG | |||
ESR2 (ERβ) | NM_207707.1 | fw | ACCCTCACTGGCACGTTGCG | 204 bp |
rev | GGCTTGCGGTAGCCAAGGGG |