Skip to main content

Table 2 qPCR data quality summary

From: Monitoring urban beaches with qPCR vs. culture measures of fecal indicator bacteria: Implications for public notification

Data quality metric 2015 2016
Accuracy in composite standard curves
Number of standard curves 9 9
R2 0.996 0.999
Amplification efficiency 1.933 2.097
Precision: calibrator samples CV
Number of samples 55 71
Enterococci target CV 2.05% 1.33%
SPC target CV 1.11% 1.41%
Inhibition Frequency Percent Frequency Percent
ΔCtSPC < 2 536 99.26 1210 96.3%
2 ≤ ΔCtSPC < 3 2 0.37% 32 2.5%
ΔCtSPC ≥ 3 2 0.37% 14 1.1%
Total 540 100% 1258 100%
  1. CV coefficient of variation