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Table 1 Experimental plan

From: The Ramazzini Institute 13-week study on glyphosate-based herbicides at human-equivalent dose in Sprague Dawley rats: study design and first in-life endpoints evaluation

Breeders

Offspring

     

Group

Animals

Group

Animalsa

Treatmentb

End of the experiment

Sex

No.

N.

Sex

Cohort

Compound

Dosec

Age at startd

Cohort

6-week (No.)

13-week (No.)

6-week (PND)

13-week (PND)

I

F

8

I

M

8

10

Control (drinking water)

0

GD 6

70e

120f

M

8

 

F

8

10

F + M

16

 

M + F

16

20

II

F

8

II

M

8

10

Glyphosate

US ADI

GD 6

70e

120f

M

8

 

F

8

10

F + M

16

 

M + F

16

20

III

F

8

III

F

8

10

Roundup

US ADI Glyphosate equivalent

GD 6

70e

120f

M

8

 

M

8

10

F + M

16

 

F + M

16

20

Total

M + F

48

 

M + F

48

60

     
  1. aNo more than 2 sisters and 2 brothers per litter
  2. bTest compounds are administered ad libitum in drinking water
  3. cDoses are calculated considering the Glyphosate US ADI defined as the chronic Reference Dose (cRfD) determined by the US EPA (1.75 mg/kg bw/day)
  4. dSolutions are admistered to dams starting from the 6th day of pregnancy
  5. eAnimals are treated until the landmarks of sexual development are acquired (PND 73 ± 2)
  6. fAnimals are treated from embryonic life (GD 6) indirectly from dams milk until PND 28 ± 2, then directly for 90 days after weaning (until PND 125 ± 2)