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Table 1 Experimental plan

From: The Ramazzini Institute 13-week study on glyphosate-based herbicides at human-equivalent dose in Sprague Dawley rats: study design and first in-life endpoints evaluation

Breeders Offspring      
Group Animals Group Animalsa Treatmentb End of the experiment
Sex No. N. Sex Cohort Compound Dosec Age at startd Cohort
6-week (No.) 13-week (No.) 6-week (PND) 13-week (PND)
I F 8 I M 8 10 Control (drinking water) 0 GD 6 70e 120f
M 8   F 8 10
F + M 16   M + F 16 20
II F 8 II M 8 10 Glyphosate US ADI GD 6 70e 120f
M 8   F 8 10
F + M 16   M + F 16 20
III F 8 III F 8 10 Roundup US ADI Glyphosate equivalent GD 6 70e 120f
M 8   M 8 10
F + M 16   F + M 16 20
Total M + F 48   M + F 48 60      
  1. aNo more than 2 sisters and 2 brothers per litter
  2. bTest compounds are administered ad libitum in drinking water
  3. cDoses are calculated considering the Glyphosate US ADI defined as the chronic Reference Dose (cRfD) determined by the US EPA (1.75 mg/kg bw/day)
  4. dSolutions are admistered to dams starting from the 6th day of pregnancy
  5. eAnimals are treated until the landmarks of sexual development are acquired (PND 73 ± 2)
  6. fAnimals are treated from embryonic life (GD 6) indirectly from dams milk until PND 28 ± 2, then directly for 90 days after weaning (until PND 125 ± 2)