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Table 2 Summary of the endpoints and relative monitoring time points evaluated in the study, in dams and offspring (6-week and 13-week cohorts)

From: The Ramazzini Institute 13-week study on glyphosate-based herbicides at human-equivalent dose in Sprague Dawley rats: study design and first in-life endpoints evaluation

Endpoints Time points Dams Offspring 6-week cohort Offspring 13-week cohort
Gestation length GD0-delivery
AGD and body weight in male and female pups PND 1
Litter size PND 1, 4, 7, 10, 13, 16, 19, 21, 25
Live-birth index PND 1
Survival index PND 4, 7, 10, 13, 16, 19, 21, 25
Age and body weight at BPS in male pups PND 35
Age and body weight at VO in female pups PND 28
First estrous in female pups 3 days after VO
Estrous cycle length and percentage of days in each stage PND 95 - PND 116
Estrous cycle prior to necropsy PND 125 ± 2
Serum hormone measures End of lactation (dams), PND 73 ± 2 and PND 125 ± 2
Clinical biochemistry PND 73 ± 2 and PND 125 ± 2
Urinalysis PND 73 ± 2 and PND 125 ± 2
Glyphosate and AMPA detection in urine End of lactation (dams), PND 73 ± 2 and PND 125 ± 2
Sperm counts PND 73 ± 2 and PND 125 ± 2
Daily Sperm production PND 73 ± 2 and PND 125 ± 2
Sperm transit time through the epididymis PND 73 ± 2 and PND 125 ± 2
Sperm morphology PND 73 ± 2 and PND 125 ± 2
Sperm aneuploidy PND 73 ± 2 and PND 125 ± 2
Partial histopathology (reproductive organs, brain, liver, kidney) End of lactation (dams)
Complete histopathology PND 73 ± 2 and PND 125 ± 2
Organ weight End of lactation (dams), PND 73 ± 2 and PND 125 ± 2
Micronuclei test (bone marrow) PND 73 ± 2 and PND 125 ± 2
Transcriptome on mammary glands End of lactation (dams), PND 73 ± 2 and PND 125 ± 2
Transcriptome on brain PND 125 ± 2
Transcriptome on liver End of lactation (dams), PND 73 ± 2 and PND 125 ± 2
Transcriptome on kidneys End of lactation (dams), PND 73 ± 2 and PND 125 ± 2
Microbiome analysis in dams Before mating, GD 5 (before treatment), GD 13, LD 7, LD 14
Microbiome analysis in offspring PND 7, PND 14, PND 31 (before puberty), PND 57 (after puberty), PND 125 ± 2 (adulthood)
  1. GD gestation day, LD lactation day, PND postnatal day, AGD anogenital distance, VO vaginal opening, BPS balano preputial separation