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Table 2 Effects of glyphosate or Roundup Bioflow exposure on developmental landmarks and sexual characteristics of pups

From: The Ramazzini Institute 13-week pilot study glyphosate-based herbicides administered at human-equivalent dose to Sprague Dawley rats: effects on development and endocrine system

Parameter

Control

Glyphosate

Roundup

Number of male pups at PND 1

58

46

53

Male pups weight at PND 1 (g)a

6.8 ± 0.5

7.1 ± 0.2

6.8 ± 0.4

Male pups weaning weight (g)a, b

50.4 ± 4.4

53.5 ± 6.0

51.8 ± 5.8

Male AGD (mm) at PND 4a, c

4.02 ± 0.49

4.26 ± 0.38**

4.34 ± 0.30**°°

Age (PND) at balano-preputial separation (BPS)

46.33 ± 1.85

46.78 ± 1.73

47.61 ± 2.77

Body weight at BPS (g)

202.50 ± 10.74

203.89 ± 16.68

207.50 ± 22.70

Number of female pups at PND 1

51

61

60

Female pups birth weight (g)a

6.4 ± 0.4

6.6 ± 0.4

6.5 ± 0.6

Female pups weaning weight (g)a, b

48.3 ± 5.1

50.4 ± 5.2

50.5 ± 5.1

Female AGD (mm) at PND 4a, c

1.70 ± 0.25

1.79 ± 0.21

1.86 ± 0.19**°°

Age (PND) at vaginal opening (VO)a

35.56 ± 1.72

35.39 ± 1.5

35.61 ± 1.14

Body weight at VO (g)a

108.33 ± 6.18

108.06 ± 7.10

109.44 ± 8.73

Age (PND) at First Estrous (FE)a, d

39.88 ± 1.25

40.13 ± 1.46

42.63 ± 3.25#

Number of days between VO and FEa

4.75 ± 0.71

5.13 ± 0.64

7.00 ± 3.78

  1. **Statistically significant (p < 0.01) with multilevel linear regression adjusted for body weight
  2. °°Statistically significant (p < 0.01) with multilevel linear regression adjusted for body weight and litter (random effect)
  3. #Statistically significant (p < 0.05) with Kruskal-Wallis’ tests
  4. aMean ± standard deviation
  5. bWeaning weight corresponds to PND 25
  6. cAGD = ano-genital distance
  7. dFirst estrous (FE) was evaluated only in females belonging to the 6 week cohort