Fig. 7

Determination of optimal serum volume in the SPAA. Cos-7 cells were transfected, and control wells were treated as described in Fig. 5. Experimental wells were treated with 0.5–50 μL of serum. Luminescence and fluorescence were measured after 24 h. Data were calculated as described in the Methods. (a) PPRE transcriptional activity measured in different volumes of serum (Gemini). Different letters indicate group means that differed significantly, while groups with the same letter did not differ significantly (p < 0.05, ANOVA, Tukey). (b) Dose response fit of PPRE transcriptional activity measured in different volumes of serum. Dose response data were fit with a 3-parameter sigmoid equation. Data are reported as mean ± standard error (N = 3 independent transfections)