Table 1 Reagents to perform the Serum PPARγ Activity Assay

Cos-7 cells (RRID:CVCL_0224, CRL-1651, ATCC)

Maintenance media: Dulbecco’s Modified Eagle’s Medium (DMEM) (Corning Cellgro, Tewskbury, MA) with 5% fetal bovine serum (FBS), amphotericin B/penicillin/streptomycin, and L-glutamine

96-Well White-sided Clear Bottom Plates (cat. #: 07–200-566; Corning)

Plasmids:

human PPARG1 [54] or mouse Pparg1 (plasmid 8886; Addgene)^a

PPRE-3x-Tk (plasmid 1015; Addgene)

pcDNA (plasmid 20011; Addgene)

CMV-eGFP (plasmid 11153; Addgene)^b

Lipofectamine 2000 Transfection Reagent (cat. #: 11668019; Thermo Fisher Scientific)

Opti-MEM Reduced Serum Media (cat. #: 31985062; Thermo Fisher Scientific)

Charcoal/Dextran stripped FBS (cat. #: F6765, Sigma)

Rosiglitazone (cat. #: 71740; Cayman Chemical)

T0070907 (cat. #: T8703; Sigma)

Vehicle solvent mix (50% Ethanol (cat. #: 04–355-453, Fisher Scientific): 50% Dimethyl sulfoxide (cat. #: AB03091; American Bioanalytical))

Commercial Human Serum Standard (cat. #: 100–512, Gemini Bio-Products)

Steady-Luc Firefly HTS Assay (cat. #: 30028-L3; Biotium)