Fig. 7

Mixture effects on neurite outgrowth. hiPSC-derived NSCs were differentiated for 7 DIV, and then treated for either 3 days (a, c) or 14 days (b, d) with single chemicals (BPA, CPF, Lead, Methyl-Hg, PCB138 or VA) and three types of mixtures: (i) a mixture with the 3 similar MoA chemicals (labelled ‘3-Sim’), (ii) a mixture with the 3 dissimilar MoA chemicals (labelled ‘3-Diss’), and (iii) a mixture with all 6 chemicals (labelled ‘All’). (a, b) Graphs reporting neurite length (black), the number of branch points/neurite (grey), and the number of neurites/neuron (violet curves) analysed upon treatments with LOAEC/4-neu concentrations (see Table 3). (c, d) Representative immunocytochemical image (at 10x magnification, with 40x magnifications insets showing applied masks for detection of neurites) of cells treated with neurite outgrowth-related mixtures (at LOAEC/4-neu concentrations) for either 3 days (c) or 14 days (d) and stained for β-III-Tubulin (red). All samples were normalised to medium containing solvent only (0.1% DMSO, Ctr). Data are represented as mean ± S.E.M. of 3–4 biological replicates