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Fig. 8 | Environmental Health

Fig. 8

From: Assessment of developmental neurotoxicity induced by chemical mixtures using an adverse outcome pathway concept

Fig. 8

Mixture effects on BDNF levels. hiPSC-derived NSCs were differentiated for 7 DIV, and then treated for either 3 days (A, C) or 14 days (b, d) with single chemicals (BPA, CPF, Lead, Methyl-Hg, PCB138 and VA) or three types of mixtures: (i) a mixture with the 3 similar MoA chemicals (labelled ‘3-Sim’), (ii) a mixture with the 3 dissimilar MoA chemicals (labelled ‘3-Diss’), and (iii) a mixture with all 6 chemicals (labelled ‘All’). (A, B) Graphs show BDNF total levels measured upon treatment with single chemicals or mixtures at LOAEC-bdnf (red curve, only after 3 day treatment) and their serial dilutions (respectively black (LOAEC/2-bdnf) and blue (LOAEC/4-bdnf) curves). (c, d) Representative immunocytochemical image (at 10x magnification, with 40x magnifications insets) of cells treated with BDNF-related mixtures (at LOAEC/2-bdnf concentrations) for either 3 days (c) or 14 days (d) and stained for β-III-Tubulin (red) and BDFN (green). For the analyses, all samples were normalised to solvent control (0.1% DMSO, Ctr) at the respective time point. Data are represented as mean ± S.E.M. of 3–4 biological replicates

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