Table 4 Main effects and mixture-related LOAECs specific for each time interval and DNT endpoint
3 days | ‘3-Sim’ Similar MoA | ‘3-Diss’ Dissimilar MoA | ‘All’ |
Synaptogenesis (SYP, PSD95) | ⇑ SYPt ⇑ synapses (LOAEC/4-syn) BPA, CPF | ⇑ SYPt ⇑ synapses (LOAEC/4-syn) Methyl-Hg, PCB138 | ⇑ SYPt (LOAEC/4-syn) BPA, CPF, Methyl-Hg, PCB138 |
Neurite outgrowth and % of neurons | ⇓ n. branch points ⇓ neurite length (LOAEC/4-neu) ⇑ β-III-tubulin+ neurons (LOAEC/2-neu) CPF | ⇓ n. neurites/neuron (LOAEC/4-neu) PCB138 (counteract) | ⇓ n. neurites/neuron (LOAEC/4-neu) ⇑ β-III-tubulin+ neurons (LOAEC-neu) CPF PCB138 (counteract) |
BDNF levels | ⇑ total BDNF (LOAEC-bdnf) | ⇑ total BDNF (LOAEC-bdnf) | ⇑ total BDNF (LOAEC/2-bdnf) |
14 days | ‘3-Sim’ Similar MoA | ‘3-Diss’ Dissimilar MoA | ‘All’ |
Synaptogenesis (SYP, PSD95) | ⇑ SYPt (LOAEC/2-syn) BPA | ⇔ | ⇑ SYPt (LOAEC-syn) BPA |
Neurite outgrowth and % of neurons | ⇓ neurite length (LOAEC/4-neu) ⇑ β-III-tubulin+ neurons (LOAEC/2-neu) CPF, Lead | ⇓ n. branch points ⇓ neurite length ⇑ β-III-tubulin+ neurons (LOAEC/2-neu) Methyl-Hg, PCB138, VA (counteract) | ⇓ n. branch points ⇓ neurite length ⇑ β-III-tubulin+ neurons (LOAEC/2-neu) CPF, Lead, Methyl-Hg, PCB138 VA (counteract) |
BDNF levels | ⇑ total BDNF (LOAEC/2-bdnf) CPF, Lead | ⇔ ⇑ total BDNF (LOAEC/2-bdnf) Methyl-Hg | ⇑ total BDNF (LOAEC/2-bdnf) CPF, Lead, Methyl-Hg |