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Fig. 2 | Environmental Health

Fig. 2

From: Dibutyl phthalate promotes juvenile Sertoli cell proliferation by decreasing the levels of the E3 ubiquitin ligase Pellino 2

Fig. 2

The effect of monobutyl phthalate (MBP) on the proliferation of TM4 cells. a Cell viability was measured by CCK-8 assay and showed the viability of TM4 cells after treatment with different concentrations of MBP for 5 days (n = 3). b Immunofluorescence staining showed EdU incorporation in TM4 cells without treatment (control) or following treatment with 0.1, 1, or 10 mM for 24 h. DAPI was used to counterstain cell nuclei. c Quantification of the average percentage of EdU+ cells for B (n = 6). The results are expressed as the means ± SEM. * p < 0.05; ** p < 0.01, compared with control

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Environmental Health

ISSN: 1476-069X