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Fig. 3 | Environmental Health

Fig. 3

From: Dibutyl phthalate promotes juvenile Sertoli cell proliferation by decreasing the levels of the E3 ubiquitin ligase Pellino 2

Fig. 3

The intrinsic apoptotic pathway participated in MBP-induced apoptosis of TM4 cells. a Annexin V-FITC/PI was used to stain apoptotic cells, which were analysed by flow cytometry at 24 h. b The level of apoptosis in TM4 cells was calculated (n = 3). c The protein levels of Bax and Bcl-2 in TM4 cells treated with different concentrations of MBP were measured by Western blotting; the Bax/Bcl-2 ratio was determined by ImageJ (lower panels, n = 3). GAPDH was assessed as an internal control. d Cytochrome c (Cyt c) release was detected in the cytosolic (Cytosol) fraction of MBP treated TM4 cells 24 h by Western blotting. The densitometry data were quantified with ImageJ (lower panels, n = 3). GAPDH was assessed as an internal control. The results are expressed as the means ± SEM. ** p < 0.01; * p < 0.05

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