Fig. 6

MAPK/JNK signalling is responsible for the proliferation of TM4 cells stimulated by 0.1 mM MBP. a, b Nuclear and cytosolic fractions were prepared from control and MBP-induced TM4 cells. Levels of c-Jun were analysed by Western blotting. GAPDH and Lamin B1 served as cytosolic and nuclear markers, respectively. The expression levels of c-Jun in the nucleus and cytosol were quantified with ImageJ (right panel; n = 3). c, d TM4 cells were pretreated with SP600125 for 1 h, which was followed by 24 h of treatment with 0.1 mM MBP. (c) Expression levels of c-Jun, p-c-Jun, JNK, p-JNK and cyclin dependent kinase 1 (CDK1) were determined by Western blotting. (d) The densitometry data were quantified with ImageJ (n = 3). GAPDH was run as an internal control. e, f Apoptotic cells were determined by flow cytometry. The level of apoptosis in TM4 cells was calculated (n = 3). The data are expressed as the means ± SEM. ** p < 0.01; * p < 0.05, vs control. # p < 0.05, vs MBP exposure