Fig. 5
From: Sex-dependent dysregulation of human neutrophil responses by bisphenol A
Representative images of NETs induced in vitro by LPS, BPA, or E2 in human neutrophils. Neutrophils from healthy donors (women and men) were stimulated with the indicated reagents and analyzed by a fluorescence microscope and In Cell Analyzer Workstation (c–differential interference contrast setting). Cells were stained with Hoechst 3342 against chromatin (a–blue) and anti-MPO antibody (b–green) to verify the NET production. Original magnification × 20. Representative images of neutrophils are shown. 1–unstimulated neutrophils; 2–LPS (10 μg/ml) stimulation; 3–BPA (16 nM) stimulation; 4–BPA (1.6 μM) stimulation; 5–E2 (100 pM) stimulation