Sex-dependent dysregulation of human neutrophil responses by bisphenol A

Table 4 Summary of the effect of BPA or E2 on neutrophil functions

Function	Test	Tested substance
		BPA (16 nM)		BPA (1.6 μM)		E2 (100 pM)
		Women	Men	Women	Men	Women	Men
Chemotaxis	Boyden chamber	↓	↓	↓	↓	↔	↔
Phagocytosis	Park’s method with latex (%)	↔	↔	↔	↔	↔	↔
Phagocytosis	Park’s method with latex (SCORE)	↔	↔	↑	↓	↔	↔
NADPH oxidase activity	Park’s test with NBT/NBT spontaneous	↔	↔	↔	↑	↔	↔
NADPH oxidase activity	Park’s test with NBT/NBT stimulated	↑	↔	↑	↔	↑	↔
NETs	Fluorescence microscopy	↑	↑	↑	↑	↔	↔
CD284 (TLR4)	Flow cytometry	↑	↑	↑	↑	↔	↔
CD14	Flow cytometry	↑	↑	↔	↔	↔	↔
NO	Griess reaction	↑	↑	↑	↑	↑	↑

Legend: ↑—increase, ↓—decrease, ↔—no statistical change. Abbreviations: BPA bisphenol A, E2 17β-estradiol, NETs neutrophil extracellular traps, NO nitric oxide, NBT nitroblue tetrazolium, SCORE Social Cohesion and Reconciliation

ISSN: 1476-069X