Fig. 3

Perinatal exposure to DDTs alters metabolism in 4-month-old female mice. Indirect calorimetry measurements for a heat and b oxygen consumption following IP injection with β3AR agonist CL 316,243 (0.1 mg/kg) or PBS, recorded and averaged from minute 11 to minute 31 post-injection. Data presented as mean ± SE, n = 7 animals per group. Indirect calorimetry-derived parameters were modeled with the fixed effects of perinatal exposure, acute (agonist or Veh) exposure, and their interaction, while accounting for the repeated measures from individual mice and resulting covariance structure in a mixed linear model that allows the data to exhibit within- individual correlation (PROC MIXED, SAS v9.4). Significance thresholds were p < 0.05 for main effects and p < 0.1 for interaction effects, vs Veh. ***p < 0.001; **p < 0.01. DDT, dichlorodiphenyltrichloroethane