Fig. 6

Perinatal DDTs and p,p’-DDE exposure decreased synaptic density in stellate ganglia of 4-month-old female mice. a Representative images of stellate ganglia stained for synaptic puncta, identified by co-localization of the presynaptic marker Bassoon (Bas, green) and the postsynaptic marker PSD93 (red). Scale bar = 14 μm. b Quantification of mean synaptic puncta per ROI, with n = 6–9 ROIs averaged per sample and 4–5 animals per treatment used to calculate the mean. Data presented as mean ± SE. Statistical significance determined by one-way ANOVA with post hoc Tukey’s test. *p < 0.05 vs Veh. Veh, vehicle (organic olive oil); DDT, dichlorodiphenyltrichloroethane; DDE, dichlorodiphenyldichloroethylene; Bas, Bassoon; PSD93, postsynaptic density 93