Open Access
Open Peer Review

This article has Open Peer Review reports available.

How does Open Peer Review work?

Prenatal chlorpyrifos exposure alters motor behavior and ultrasonic vocalization in cd-1 mouse pups

  • Aldina Venerosi1Email author,
  • Laura Ricceri1,
  • Maria Luisa Scattoni1 and
  • Gemma Calamandrei1
Contributed equally
Environmental Health20098:12

DOI: 10.1186/1476-069X-8-12

Received: 04 December 2008

Accepted: 30 March 2009

Published: 30 March 2009



Chlorpyrifos (CPF) is a non-persistent organophosphate (OP) largely used as pesticide. Studies from animal models indicate that CPF is a developmental neurotoxicant able to target immature central nervous system at dose levels well below the threshold of systemic toxicity. So far, few data are available on the potential short- and long-term adverse effects in children deriving from low-level exposures during prenatal life and infancy.


Late gestational exposure [gestational day (GD) 14–17] to CPF at the dose of 6 mg/kg was evaluated in CD-1 mice during early development, by assessment of somatic and sensorimotor maturation [reflex-battery on postnatal days (PNDs) 3, 6, 9, 12 and 15] and ultrasound emission after isolation from the mother and siblings (PNDs 4, 7 and 10). Pups' motor skills were assessed in a spontaneous activity test on PND 12. Maternal behavior of lactating dams in the home cage and in response to presentation of a pup previously removed from the nest was scored on PND 4, to verify potential alterations in maternal care directly induced by CPF administration.


As for the effects on the offspring, results indicated that on PND 10, CPF significantly decreased number and duration of ultrasonic calls while increasing latency to emit the first call after isolation. Prenatal CPF also reduced motor behavior on PND 12, while a tendency to hyporeflexia was observed in CPF pups by means of reflex-battery scoring. Dams administered during gestation with CPF showed baseline levels of maternal care comparable to those of controls, but higher levels of both pup-directed (licking) and explorative (wall rearing) responses.


Overall our results are consistent with previous epidemiological data on OP neurobehavioral toxicity, and also indicate ultrasonic vocalization as an early marker of CPF exposure during development in rodent studies, with potential translational value to human infants.


The OP chlorpyrifos is a non-persistent insecticide widely employed in domestic, agricultural and non-agricultural (i.e. schools, golf courses, parks) settings. Its toxicity, related to inhibition of brain and systemic acetylcholinesterase (AChE), is well documented after acute poisoning of adults. The evaluation of CPF neurotoxicity after sub-toxic exposure and in developing organisms appears more controversial, as most of available animal studies indicates that CPF exposure below the threshold for systemic toxicity exerts disruptive effects on CNS development and behavior [112]. In the last decade, increased concern has been raised about adverse effects of pesticides on central nervous system (CNS) development [13, 14]. Prolonged exposure, multiple ways of exposure, and exposure to mixture of pesticides could indeed determine – also at apparently sub-toxic doses – a level of CPF burden compatible with increased health risk. The US Environmental Protection Agency (EPA) imposed a ban on its sale for residential use [15], thus the use of CPF in the USA has been restricted to agricultural applications only. However, agricultural and non-agricultural use remains of some concern and the final report of Interim Reregistration Eligibility Decision foresees mitigation measures to reduce some occupational and ecological exposures by eliminating use sites and reducing application rates [16]. In Europe, regardless of the wide and frequently OPs use, with CPF the top selling insecticide [17], no restrictions of use site or application rate are currently required [18].

A recent review [19] summarizes epidemiological studies that support the developmental neurotoxicity of OPs, although limitations of the available data were overtly admitted. In the CHAMACOS cohort study, including women resident in an area of major agricultural production, the presence of the OP metabolite dialkylphosphate (DAP) in maternal urine or blood was associated with impaired reflex functioning in infants after PND 3 [20]. Similar data are reported in a birth cohort study from New York City [21]. Impairment in mental and psychomotor performance and attention problems in infants assessed at 12, 24, and 36 months were found to be associated with CPF levels in the cord blood in a longitudinal birth study of inner-city mothers [22]. Comparable behavioral problems were reported in the CHAMACOS cohort in 24-month-old children [23].

Despite results from epidemiological studies indicate that some effects of developmental exposure to CPF are already evident in early infancy, few rodent studies so far have focused on the behavioral effects of CPF in the early developmental phases. In preweaning rats righting reflex and cliff avoidance tests were markedly altered following repeated, low-level CPF exposures during late gestation [24]. Deficits in righting reflex and geotaxis response were also reported in rat female pups after PND 1–4 exposure [6]. In a mouse model of gene-environment interactions, prenatal chlorpyrifos exposure per se induced an accelerating effect on maturation of grasping reflex in mutant Reeler mice [25].

Altricial species, such as rodents, may represent a useful animal model to mimic the immature development of body and motor skills in humans at birth [26]. In rodents several reflexes and behavioral responses show a remarkable regularity in their time of appearance and subsequent maturation, thus representing a reliable tool for assessing abnormalities in early neurodevelopment [27, 28]. Batteries of developmental milestones have been designed to describe early neurodevelopment of newborn rodents and include behavioral markers of maturation of proprioception (tactile response such as grasping, placing etc), and vestibular function which involves acquisition of coordination and adequate strength [29, 30]. The ontogenetic profile of rodent neurobehavioral development can also be measured by the analysis of the species-specific emission of ultrasound vocalizations (USVs) which are characterized by frequencies ranging from 30 to 90 kHz. USVs present a clear ontogenetic profile peaking around day eight after birth and decreasing close to zero when pups are 2-weeks old [3133], and are elicited in neonate rodents by several environmental and pharmacological stimuli [34, 35]. Several experimental evidences support USVs as indicators of the emotional state of neonate rodents [36, 37] with an important role in the establishment of the mother – offspring bond [38].

In this study we evaluate the effects of late gestational CPF exposure (GD 14–17) on early neurobehavioral development in mouse pups in order to extend our previous studies on CPF developmental exposure, that were principally focused on long-term neurobehavioral effects. The dose of CPF selected (6 mg/kg) does not elicit systemic toxicity in pregnant females and their offspring and fails to inhibit brain AChE of pups at birth as previously shown using the same CPF dose, and this same treatment schedule in the CD-1 mouse strain [10]. Throughout the first fifteen postnatal days, analysis of sensorimotor reflex maturation was carried out, and the ontogenetic profile of USV emission after isolation from the mother was assessed on PND 4, 7, and 10. Our interest towards USV emission was threefold: 1) USV are one of the few early markers of neurobehavioral development in rodent models; 2) we found in our previous studies on developmental CPF exposure long-term alterations in behaviors that reflect emotional/affective states in mice, such as agonistic behavior in adult males [10], maternal aggression [12], social novelty [11] and maternal behavior [10, 12], and USV are an appropriate end point to assess emotional responses early in life; 3) developmental CPF exposure is reported to interfere with the expression of serotonergic receptors as well as with serotonin turn-over [2, 8], and to increase oxytocin hypothalamic levels [39]. Ultrasonic calls are modulated both by serotonergic and oxytocinergic neurotransmission [4043]. We also analyzed spontaneous behavior of pups on PND 12, to evaluate overall level of activity and quality of the age-specific motility repertoire. In addition, in order to evidence potential changes in females' behavior induced by gestational CPF administration, a selection of maternal responses of lactating females was assessed when offspring were 4-days old.


Animals and Treatments

All experiments on animals were performed according to the European Community Council Directive 86/609/EEC and to Italian Legislation on Animal Experimentation (Legislative Decree 116/92). Male and female mice of a Swiss-derived outbreed strain (CD-1, Harlan, S. Pietro al Natisone, Italy), were housed in breeding cages with a 12-hr light-dark cycle (light on 20:00-8:00) and with free access to food and water. Females were inspected daily for the presence of the vaginal plug (GD 0). The stud was removed 10 days after the discovery of the vaginal plug. On GD 15 forty females were randomly assigned to one of the two prenatal treatments [vehicle (Veh), CPF]. CPF (Chem. Service, West Chester, PA) was dissolved in peanut oil (Veh) to provide rapid and complete absorption. CPF (in a volume of 0.1 ml/10 g at a dose of 6 mg/kg) or its vehicle was administered to pregnant females from GD 15 to 18 by intraoral gavages. We have previously shown a 75% serum AChE inhibition 24 hr after termination of same CPF treatment in pregnant females, while brain AChE activity was reduced to 60% of control values, an effect no longer detectable 24 h later [10]. However, the observed AChE inhibition was not associated with any sign of systemic toxicity and/or weight loss in pregnant females or altered reproductive performances (fetus viability, number of pups delivered, sex ratio and mean pups' weight at birth). Furthermore, this CPF treatment schedule did not affect brain AChE activity in offspring, but causes a mild transient inhibition (20% of control values) in serum AChE activity at birth [10].

Thirty-four litters (18 Vehicle-treated and 16 CPF-treated) were used and culled at birth to five males and five females. Assessment of sex of the pups was done by evaluation of anogenital distance [44]. On the day of birth, three pups were tattooed with permanent ink on their limbs for individual identification and randomly assigned to one of the following experiment: sensorimotor assessment and spontaneous behavior (one male and one female from each litter), USV recording (one male). The remaining pups were left undisturbed till weaning and subsequently assigned to a different experiment.

Dams' behavior

A selection of maternal responses expressed by individual dams (Veh, n = 15; CPF, n = 15) was recorded on post-partum day 4. All observations (about 10 minutes each) were made under red light in an experimental room separated from the animal colony and kept in standard environmental conditions. The observations were made between 10:00 and 14:00 hr and were performed by a trained observer blind to the assignment of the dams to the different treatment groups. Each observation consisted of two sessions: Session 1 (8 min): after removal of one male pup [this pup was concomitantly evaluated for USV in a sound proof experimental room (see below)]. Session 2 (2 min): after this same male pup was returned to the home cage.

The following behavioral categories were considered: Pup-directed behaviors – Retrieving: the female is picking up the pup in her mouth and carrying it to the nest; Licking: the animal is licking any part of the pup's body, primarily the anon-genital region; Sniffing: the female is sniffing one or more pups; Crouching: the female's body is arched over the pups with no other apparent movement. Non pup-directed behaviors – Digging: digging in the sawdust out of nest side, pushing and kicking it around using the snout and/or both forepaws and hind paws, in most cases the animals are moving around the cage, sometimes changing the whole arrangement of the substrate material; Self-grooming: the female is wiping, licking, combing or scratching any part of its own body; Wall Rearing: the female rears on hind limbs, while leaning with the forelimbs on the cage walls (or not), often sniffing the air; Locomotion: body movements assessed by number of crossing of two virtual lines dividing the cage in three sectors on the screen during videotape analysis; Resting.

Frequency and duration of each behavioral category were collected using The Observer software (Noldus, Wageningen, NL) by an individual blind to the experimental conditions.

Assessment of Somatic and Behavioral Development (PNDs 3, 6, 9, 12 and 15)

On PND 3, 6, 9, 12, and 15, one male and one female from each litter in each treatment group (Veh n = 18 M, 18 F; CPF n = 16 M, 16 F) were assessed for somatic and neurobehavioral development.

Pups were weighed to the nearest 0.01 g and their body was measured by a flexible rule to the nearest 0.1 cm. Hair growth, day of eyelid opening, and incisor eruption were also recorded.

Pups were then assessed for a number of measures currently used in the study of sensorimotor ontogeny in mice according to a slightly modified Fox battery [25, 29, 45]. The tests were conducted during the dark period between 09:00 and 14:00 hr under red light, each subject being tested at approximately the same time of the day. The following reflexes and responses were considered:

Quantitative data

Righting reflex: pup turns over with all four feet on the ground when placed on its back. The reflex was tested three times with a cut-off latency of 30 s. Data for the best performance (shorter latency) and data for the worst performance (longer latency) were analyzed separately; Grasping reflex: to evaluate the strength of grasping reflex, pups were placed on a narrow mesh metal grid in an horizontal position. The grid was progressively tilted to a vertical position (+ 90°) and further to a horizontal upside-down position (+ 180°), the angle of the grid at which pup falls is termed "fall angle". The test was repeated thrice, and the mean angle reached was recorded; Cliff aversion: pup withdraws from the edge of a flat surface when its snout and forepaws are placed over the "cliff", time of aversion was recorded with a cut-off of 10 s.

Qualitative data

Forelimb and hind limb placing reflexes: pup raises and places its fore- or hind paw on the surface of the edge of an object when stroked on the dorsum of the paw; Forelimb and hind limb stick grasp reflexes: pup grasps a toothpick when the fore- or hind paw is stroked; Pole grasping: pup grips a wooden pencil with its forepaws; The following scores were used for qualitative somatic and behavioral variables so far mentioned: 0 = no response; 1 = uncertain response; 2 = incomplete response; 3 = full response.

Ultrasonic Vocalization (PNDs 4, 7, 10)

Ultrasonic calls of one male from each litter in each treatment group (Veh n = 15; CPF n = 15) were recorded in a sound-attenuating chamber (Amplisilence, I-10070 Robassomero, Italy) during the dark period between 10:00 and 14:00 hr. Single pups were removed from the home cage and individually placed in a glass container (diameter 5 cm, height 10 cm). The number of ultrasonic calls emitted during the 4 min test was assessed using an ultrasonic microphone (Avisoft UltraSoundGate condenser microphone capsule CM16, Avisoft Bioacoustics, Berlin, Germany) sensitive to frequencies between 10–180 kHz was suspended 10 cm above the glass. Vocalizations were recorded using an Avisoft Recorder (Version 3.2). Settings included sampling rate at 250 kHz; format 16 bit. For acoustical analysis, recordings were transferred to Avisoft SASLab Pro (Version 4.40) and a fast Fourier transformation (FFT) was conducted. Spectrograms were generated with an FFT-length of 512 points and a time window overlap of 75% (100% Frame, Hamming window). The spectrogram was produced at a frequency resolution of 488 Hz and a time resolution of 1 ms. A lower cut-off frequency of 15 kHz was used to reduce background noise outside the relevant frequency band to 0 dB. Call detection was provided by an automatic threshold-based algorithm and a hold-time mechanism (hold time: 0.005 s). An experienced user checked the accuracy of call detection, and obtained a 100% concordance between automated and observational detection. Parameters analyzed included for each test day number of calls, duration of calls, frequency (kHz) and amplitude at maximum of the spectrum.

At the end of the 4 min recording session, the axillary temperature of each pup was measured by gentle insertion of the thermal probe in the skin pocket between upper foreleg and chest of the animal for about 30 s (Microprobe digital thermometer with mouse probe, Stoelting Co., Illinois, USA).

Spontaneous motor behavior (PND 12)

One male and one female pup from each litter assigned to the two experimental conditions were assessed for their spontaneous motor behavior on PND 12 (Veh, n = 13 M, 13 F; CPF, n = 14 M, 14 F). All observations (one session of 5 min) were made under red light in an experimental room separated from the animal colony and kept in standard environmental conditions. The observations were made between 10:00 and 14:00 hr. Pups were individually introduced in a glass cylinder (14 cm diameter, 8 cm height) with adsorbent paper on the floor kept in an incubator set at 30 ± 1°C.

The following behavioral items were collected: Crossing (forward movements of the body); Immobility (no visible movements of the body); Head moving (head raising and head turning); Wall climbing; Pivoting (turning to left or right propelled only by forelimbs; hind limbs stationary); Grooming (signs of wiping).

Frequency and duration of each behavioral category were collected using The Observer software (Noldus, Wageningen, NL) by an experimenter blind to the experimental condition of the animals.

Statistical analysis

Parametric analysis of variance (ANOVA) was performed on frequency and duration of dams' behavior responses, USV data, pups' body weight and length, Grasping, Righting and Cliff avoidance data. Specifically: ANOVA model for maternal behavior data included prenatal treatment (2 levels) as between-subject fixed factor; ANOVA model for USV data included prenatal treatment (2 levels) as between-subject fixed factor and days as repeated measure factor (3 levels); ANOVA model for pups' body weight and length, Grasping, Righting and Cliff avoidance reflexes included prenatal treatment (2 levels) as between-litter fixed factor, litter as random blocking factor nested within prenatal treatment and blocking factor for sex and days, sex as within-litter fixed factor (2 levels) and day as repeated measure factor (5 levels).

Posthoc comparisons were performed using Tukey's HSD test, which can be used in the absence of significant ANOVA results [46].

Non-parametric analyses of variance (ANOVAs) were performed on qualitative data for sensorimotor maturation: for each reflex a synthetic measure of the score profile across days was computed (area under the curve generated by the repeated scores) and Mann-Whitney U test was applied. Moreover, in order to obtain information on differences in maturation level across days, a Total Daily Score was computed for each subject in each day of testing, by summing the scores of all reflexes recorded on that subject in that day. The Total Daily Score was analyzed by parametric ANOVA, including prenatal treatment (2 levels) as between-litter fixed factor, litter as random blocking factor nested within prenatal treatment and blocking factor for sex and days, sex as within-litter fixed factor (2 levels) and day as repeated measures factor (5 levels). The Mann-Whitney U test was also performed day-by-day. When performing the Mann-Whitney U test (for both area under the curve and Total Daily Score), the response variable was transformed by summing the observations on the two subjects (one male and one female) per litter, to assess main effect of prenatal treatment, and by computing the difference between the observations from the same two subjects, to assess the interaction prenatal treatment x sex. Data are reported by means ± SEM.


Reproductive performance

On PND 0 data analysis on number of delivered pups (Veh: 11.5 ± 0.5 CPF: 10.2 ± 0.7); sex rate (% males Veh: 41.6 ± 2.6, CPF: 42.3 ± 3.6; % females Veh: 57.0 ± 2.8, CPF: 51.8 ± 4.1) and overall litter weight (Veh, 20.19 ± 0.91 g; CPF, 19.81 ± 1.15 g) did not show any detrimental effect of gestational CPF exposure.

Dams' behavior

CPF gestational exposure did not affect maternal responses recorded during Session 1 (data not shown).

During Session 2, latency to retrieve the male pup returned to the home cage was not altered by CPF. As for Licking response towards all the pups, CPF tended to increase its duration [F (1, 28) = 3.44 p = 0.07] (see Figure 1). The other pup-directed maternal behaviors were not affected by CPF during Session 2. As for non pup-directed behaviors, CPF significantly increased Wall rearing [duration: F (1, 28) = 7.85 p = 0.01; frequency: F (1, 28) = 3.99 p = 0.05] and decreased Digging duration [F (1, 28) = 6.46 p = 0.01].
Figure 1

Dams' Behavior. Selected pup-directed and non pup-directed behaviors displayed by Veh and CPF treated females on postpartum day 4, after the pup was returned to the home cage. Session lasted two minutes. * p < 0.05. Veh, n = 15; CPF, n = 15.

Assessment of Somatic and Neurobehavioral Development (PNDs 3, 6, 9, 12, and 15)

A significant treatment x sex interaction was found for Body length [F (1, 32) = 8.67 p < 0.01], with CPF males being shorter than Veh controls (post hoc comparisons p < 0.05, see Table 1). As for body weight gain neither a main effect of CPF nor a CPF interaction with treatment, sex or day was found.
Table 1

Somatic and reflex maturation of pups prenatally exposed to Veh or CPF (6 mg/kg).


Body Lenght (cm)









3.04 ± 0.03

3.34 ± 0.03

3.67 ± 0.04

4.06 ± 0.07

4.54 ± 0.08



2.97 ± 0.05

3.27 ± 0.04

3.61 ± 0.05

3.86 ± 0.06

4.29 ± 0.08



3.02 ± 0.04

3.36 ± 0.04

3.61 ± 0.04

4.04 ± 0.06

4.43 ± 0.08



3.00 ± 0.04

3.38 ± 0.04

3.64 ± 0.04

4.02 ± 0.07

4.44 ± 0.09


Body Weight (g)









2.78 ± 0.06

4.61 ± 0.08

6.51 ± 0.18

7.84 ± 0.23

9.12 ± 0.25



2.88 ± 0.06

4.67 ± 0.12

6.45 ± 0.19

7.63 ± 0.22

8.94 ± 0.22



2.77 ± 0.06

4.39 ± 0.13

6.14 ± 0.18

7.74 ± 0.22

9.06 ± 0.23



2.77 ± 0.09

4.57 ± 0.12

6.39 ± 0.12

7.71 ± 0.18

8.98 ± 0.20


Total daily qualitative score









2.78 ± 0.17

5.28 ± 0.16

8.66 ± 0.18

12.44 ± 0.22

18.44 ± 0.22



2.53 ± 0.12

5.09 ± 0.14

8.69 ± 0.15

11.97 ± 0.24

17.50 ± 0.29

Data are indicated as (Mean ± SEM).

* p < 0.05 after posthoc comparisons, see Results

As for quantitative data on sensorimotor coordination development, CPF did not affect Grasping, Righting and Cliff aversion reflexes.

Non-parametric analysis conducted on each reflex score profile across days only evidenced a trend towards delayed appearance of Hind limb grasping in CPF exposed pups [Mann-Whitney U (18,16) = 90.5 p = 0.06]. As for the Total Daily Score, sum of the single qualitative daily reflex scores, parametric analysis showed a main effect of treatment (mean data shown in Table 1) just missing statistical significance [F (1, 30) = 3.65 p = 0.06] and no interaction of treatment with sex or day; the non-parametric analysis, conducted on the Total Daily Score in each day, evidenced a lower score in CPF exposed pups on PND15 [U (16, 16) = 79 p = 0.06].

Ultrasonic Vocalization (PNDs 4, 7, 10)

A significant interaction between CPF and day was found for number and duration of ultrasonic calls [F (1, 56) = 4.65 p = 0.01; F (1, 56) = 3.56 p = 0.03 respectively]. Post hoc comparisons showed that CPF decreased both number and duration of calls of CPF male pups on PND 10 (Ps < 0.05). Peak frequency of calls was significantly increased in CPF pups on PND 10 [treatment x day interaction: F (2, 56) = 2.41 p = 0.09, p < 0.05 after post hoc comparisons], while no effect was observed for Peak amplitude. Finally, a significant CPF delaying effect was found for the emission of the first call [U (15, 15) = 65 p = 0.04] on PND 10 (see Figure 2).
Figure 2

Ultrasound vocalization parameters. Quantitative ultrasound vocalization data recorded on PND 4, 7, and 10 during a 4- min session. * p < 0.05, ** p < 0.01. Inset graph (upper panel): Latency to emit the first call on PND10, data are median ± interquartile range. Veh n = 15 (only males); CPF, n = 15 (only males).

Spontaneous behavior (PND 12)

CPF did not interfere with the overall level of locomotor activity measured by number of crossings. However, CPF pups displayed significantly less Pivoting behavior [F (1, 25) = 5.82 p = 0.02, F (1, 25) = 5.19 p = 0.03 for frequency and duration respectively,] than control pups and showed a parallel increase of Immobility [F (1, 25) = 4.28 p = 0.05, F (1, 25) = 4.67 p = 0.04 for frequency and duration respectively] (see Figure 3).
Figure 3

Spontaneous behavior of 12-day-old pups. Behavioral responses recorded during the Spontaneous behavior test on PND 12. As for Pivoting and Immobility behavior a main effect of the treatment was found (p < 0.05; see Results section). Veh, n= 13 (both males and females); CPF, n = 14 (both males and females).


Overall the results of the present study show that administration of CPF in late gestation results in early behavioral alterations in mouse pups. The assessment of neurodevelopment across the first 15 days after birth indicates a significant depressive effect of CPF on pup behavior after day 10th of postnatal life that concerns both distress response to isolation from the mother and motor skills.

The analysis of somatic development indicates that CPF failed to affect body weight but reduced body length in male pups. This effect, that is suggestive of a subtle growth delay, is in partial agreement with what reported by one epidemiological study [47] evidencing decreased somatic growth at birth in neonates born from urban minorities women in New York City, associated with cord plasma CPF levels.

As for sensorimotor development, only a general trend towards hyporeflexia was shown by CPF mice when considering either a total score of maturation or single reflex analysis (hind limb grasping). Indeed, all effects on reflexes remain at the margin of significance and thus lying in the uncomfortable range where definitive conclusions about a retarding CPF effect cannot be drawn. In the rat species perinatal CPF administration at a dose comparable with the one used in the present study was found to delay early somatic and sensorimotor development of the offspring [24, 48]. However in these two rat studies CPF was administered for longer periods than in the present mouse study. Thus it is possible that only when CPF exposure covers a large portion of gestation it induces statistically detectable neurodevelopmental delays. In humans too abnormal reflexes have been reported after prolonged exposure, namely either in infants born from mothers exposed to different OPs from a urban cohort [21], and from a Californian cohort of farm working women [20].

The clear depressive effect of prenatal CPF on ultrasonic vocalizations observed in 10-day-old pups represents the main finding of the present study. CPF exposure resulted in lower reactivity to isolation (higher latency to emit the first call), decreased number and duration of vocalizations, and finally in a higher peak frequency of the calls. Furthermore, the altered profile of ultrasound emission was accompanied by significant changes in spontaneous motor behavior on PND 12: CPF-exposed pups displayed less Pivoting – a typical neonatal motor pattern in rodents – associated with a higher level of immobility. It is worth noting that a selective impairment in early motor reflexes, such as crawling, has been observed in newborns exposed prenatally to OPs [20, 21].

A large body of data define USV emission as a phenomenon that may contribute both to maintenance of stable body temperature and homeostasis [49], playing at the same time a communicative role in stimulating the mother to look for and retrieve the pup, thus functioning as an indicator of the affective state of the pup [50]. In a translational perspective, it is remarkable that rather similar hypotheses have been drawn to interpret human infant cry. Organs and structures (larynx, thoracic and abdominal muscles etc.) and neurological systems which modulate infant vocalizations (vagal nerve, brainstem and limbic system) are widely shared in mammals [34, 51, 52]. A large number of studies on rodent models clearly demonstrate that USVs are a sensitive (and almost unique) and reliable marker to detect short-term neurotoxic effects of developmental exposure to environmental contaminants [5355]. USVs are also sensitive to pre- and peri-natal neurotoxic effects of psychoactive drugs [56, 57].

Our results show alterations in selective features of pup vocalizations which parallel some of those currently adopted to analyze infant cry, including number and duration of calls (utterance), peak frequency (hyperphonation), and latency to emit the first call. These results thus candidate rodent neonatal USVs as an early marker in preclinical studies and potential predictors of CPF long-term effects on emotional and social competencies already reported at adulthood [1012]. In addition, the present results support the use of acoustic cry analysis in the screening for the effects of developmental exposure to OP pesticides in humans. Selected cry features have been associated with condition of potential neurological insults such as prematurity [58], developmental exposure to metals [59], and substance of abuse such as alcohol and cocaine [60, 61]. More importantly, in human infants atypical cry has been also reported concomitantly with poor performance on Brazelton-modified scale [61], a neurological assessment methodology analogues to the neurological reflex battery also applied to mouse pups in the present study [29].

Although we can not exclude that alterations in ultrasonic vocalization resulting from CPF exposure are also present later on in development, our results show a peculiar age profile with a significant decrease only on PND 10 and absence of effects on PND 4 and 7; in other words the impact of the prenatal CPF exposure was detectable only during the second postnatal week and not before, i.e. closer to time of exposure. We can only speculate on the biological mechanisms underlying the peculiar time course of prenatal CPF effects on USVs. Neonatal USV response is modulated by multiple neurotransmitter systems, and both the cholinergic [35, 62] and the serotonergic circuitries have been involved in USVs. Both these two neurotransmitter systems are considered primary targets of developmental CPF exposure at doses not involving significant inhibition of AChE activity [1, 63, 64]. However, cholinergic and serotonergic manipulations differently affect the USV profile, with effects detectable at all neonatal ages considered, as early as PND 3 for serotonergic [65] and PND 5 for cholinergic agents [35].

USVs emission in neonatal rodents is also modulated by the hypothalamic neuropeptides oxytocin (OT) and vasopressin (AVP), as demonstrated by exogenous administration of the neuropeptides and their antagonists in neonatal rat pups [40, 66], and by USVs recorded in OT and AVP 1b receptor knockout (V1b ko) mice [41, 43].

Two lines of evidence candidate these hypothalamic neuropeptides as potential neural targets for the observed CPF effects on USVs in 10-days old animals. Firstly, we have recently shown that the same dose of prenatal CPF used in the present study induces long-term enhancement of OT protein levels in the hypothalamus, and an associated decrease of AVP expression in the same brain area, with males presenting the most intense effect [39]. Secondly, data concerning the profile of OT receptor during the early phases of postnatal development clearly indicate a peak of expression starting from the second postnatal week in different brain regions [42], a profile also confirmed by recent behavioral data concerning OT modulation of huddling response in 10-day-old but not in 7-day-old rats [67]. Further research on neuropeptidergic CNS levels in pups developmentally exposed to CPF is needed to assess if the changes in OT and AVP observed in adults exposed prenatally to CPF are already detectable in the first two weeks of postnatal life and are then associated with USV changes, or if such deficit in vocalizations is a component of a general delay in motor activation as signaled by the alteration in spontaneous motor behavior.

Finally, our data suggest for the first time behavioral changes in females administered with CPF during pregnancy, in the absence of overt toxicity signs. CPF did not alter the time to retrieve the male pup, but increased the level of exploratory activity (Wall Rearing). Such activation may be a delayed effect of the transient inhibition of brain AChE, even if at the time of testing four days had elapsed from the last CPF administration, and AChE brain activity levels were back to control levels (see [10]). CPF tended to increase licking after the reunion with the male pup, but this effect was likely due to a more active response to novelty, rather than to alteration of specific aspects of maternal care. Alterations in pups' licking has been evidenced in females developmentally exposed to CPF, in the absence of significant effects on general maternal responsiveness [10, 12]. A more detailed analysis of how CPF interferes with the unique metabolic and endocrine condition represented by pregnancy and with the establishment of the mother-offspring bond appears warranted.


Overall, results from this study show that a prenatal CPF exposure restricted to 4 days in late gestation is sufficient to determine a poor behavioral outcome characterized by diminished responsiveness to a distress condition and reduced motor activity. The specificity of the behavioral responses affected by CPF in the present study confirms that developmental CPF targets, among the others, behavioral domains involved in the regulation of affective states, a feature previously reported both in rat and mouse studies at adulthood. A further important aspect is the translational value of our results, as the changes in USV patterns in rodents suggest to clinicians to consider the pattern of infant cry, further to maturation of sensorimotor reflexes, as early marker for the evaluation of the risk associate to developmental OP exposure.






analysis of variance


Central Nervous System






Environmental Protection Agency


gestational day








postnatal days


ultrasound vocalizations


United States







This research was supported by the HENVINET, Health and Environment Network, (EC Contract 037019) and by ISS/NIH project 5301/530H "Neurobehavioral phenotyping of genetically modified mouse models of mental retardation". The authors wish to acknowledge Giovanni Dominici for his technical assistance and Angela Rungi for helping in data collection.

Authors’ Affiliations

Section of Neurotoxicology and Neuroendocrinology Department of Cell Biology and Neuroscience, Istituto Superiore di Sanità


  1. Aldridge JE, Seidler FJ, Slotkin TA: Developmental exposure to chlorpyrifos elicits sex-selective alterations of serotonergic synaptic function in adulthood: critical periods and regional selectivity for effects on the serotonin transporter, receptor subtypes, and cell signaling. Environ Health Perspect. 2004, 112: 148-55.View ArticleGoogle Scholar
  2. Aldridge JE, Levin ED, Seidler FJ, Slotkin TA: Developmental exposure of rats to chlorpyrifos leads to behavioral alterations in adulthood, involving serotonergic mechanisms and resembling animal models of depression. Environ Health Perspect. 2005, 113: 527-31.View ArticleGoogle Scholar
  3. Crumpton TL, Seidler FJ, Slotkin TA: Developmental neurotoxicity of chlorpyrifos in vivo and in vitro: effects on nuclear transcription factors involved in cell replication and differentiation. Brain Res. 2000, 857: 87-98. 10.1016/S0006-8993(99)02357-4.View ArticleGoogle Scholar
  4. Dam K, Seidler FJ, Slotkin TA: Developmental neurotoxicity of chlorpyrifos: delayed targeting of DNA synthesis after repeated administration. Brain Res Dev Brain Res. 1998, 108: 39-45. 10.1016/S0165-3806(98)00028-5.View ArticleGoogle Scholar
  5. Dam K, Seidler FJ, Slotkin TA: Chlorpyrifos releases norepinephrine from adult and neonatal rat brain synaptosomes. Brain Res Dev Brain Res. 1999, 118: 129-33. 10.1016/S0165-3806(99)00139-X.View ArticleGoogle Scholar
  6. Dam K, Seidler FJ, Slotkin TA: Chlorpyrifos exposure during a critical neonatal period elicits gender-selective deficits in the development of coordination skills and locomotor activity. Brain Res Dev Brain Res. 2000, 121: 179-87. 10.1016/S0165-3806(00)00044-4.View ArticleGoogle Scholar
  7. Roy TS, Andrews JE, Seidler FJ, Slotkin TA: Chlorpyrifos elicits mitotic abnormalities and apoptosis in neuroepithelium of cultured rat embryos. Teratology. 1998, 58: 62-8. 10.1002/(SICI)1096-9926(199808)58:2<62::AID-TERA7>3.0.CO;2-2.View ArticleGoogle Scholar
  8. Slotkin TA, Seidler FJ: Prenatal chlorpyrifos exposure elicits presynaptic serotonergic and dopaminergic hyperactivity at adolescence: critical periods for regional and sex-selective effects. Reprod Toxicol. 2007, 23: 421-7. 10.1016/j.reprotox.2006.07.010.View ArticleGoogle Scholar
  9. Ricceri L, Markina N, Valanzano A, Fortuna S, Cometa MF, Meneguz A, Calamandrei G: Developmental exposure to chlorpyrifos alters reactivity to environmental and social cues in adolescent mice. Toxicol Appl Pharmacol. 2003, 191: 189-201. 10.1016/S0041-008X(03)00229-1.View ArticleGoogle Scholar
  10. Ricceri L, Venerosi A, Capone F, Cometa MF, Lorenzini P, Fortuna S, Calamandrei G: Developmental neurotoxicity of organophosphorous pesticides: fetal and neonatal exposure to chlorpyrifos alters sex-specific behaviors at adulthood in mice. Toxicol Sci. 2006, 93: 105-13. 10.1093/toxsci/kfl032.View ArticleGoogle Scholar
  11. Venerosi A, Calamandrei G, Ricceri L: A social recognition test for female mice reveals behavioral effects of developmental chlorpyrifos exposure. Neurotoxicol Teratol. 2006, 28: 466-71. 10.1016/ ArticleGoogle Scholar
  12. Venerosi A, Cutuli D, Colonnello V, Cardona D, Ricceri L, Calamandrei G: Neonatal Chlorpyrifos affects maternal responses and maternal aggression in CD-1 mice. Neurotoxicol Teratol. 2008, 30 (6): 468-74. 10.1016/ ArticleGoogle Scholar
  13. Colborn T: A case for revisiting the safety of pesticides: a closer look at neurodevelopment. Environ Health Perspect. 2006, 114: 10-7.View ArticleGoogle Scholar
  14. Grandjean P, Landrigan PJ: Developmental neurotoxicity of industrial chemicals. Lancet. 2006, 368: 2167-78. 10.1016/S0140-6736(06)69665-7.View ArticleGoogle Scholar
  15. EPA: Cancellation order. []
  16. EPA: Chlopyrifos Facts. []
  17. Eurostat: The use of plant protection products in the European Union, Data 1992 – 2003, Eurostat statistical books. []
  18. Bjorling-Poulsen M, Raun Andersen H, Grandjean P: Potential developmental neurotoxicity of pesticides used in Europe. Environ Health. 2008, 7: 50-10.1186/1476-069X-7-50.View ArticleGoogle Scholar
  19. Eskenazi B, Rosas LG, Marks AR, Bradman A, Harley K, Holland N, Johnson C, Fenster L, Barr DB: Pesticide toxicity and the developing brain. Basic Clin Pharmacol Toxicol. 2008, 102: 228-36.View ArticleGoogle Scholar
  20. Young JG, Eskenazi B, Gladstone EA, Bradman A, Pedersen L, Johnson C, Barr DB, Furlong CE, Holland NT: Association between in utero organophosphate pesticide exposure and abnormal reflexes in neonates. Neurotoxicology. 2005, 26: 199-209. 10.1016/j.neuro.2004.10.004.View ArticleGoogle Scholar
  21. Engel SM, Berkowitz GS, Barr DB, Teitelbaum SL, Siskind J, Meisel SJ, Wetmur JG, Wolff MS: Prenatal organophosphate metabolite and organochlorine levels and performance on the Brazelton Neonatal Behavioral Assessment Scale in a multiethnic pregnancy cohort. Am J Epidemiol. 2007, 165: 1397-404. 10.1093/aje/kwm029.View ArticleGoogle Scholar
  22. Rauh VA, Garfinkel R, Perera FP, Andrews HF, Hoepner L, Barr DB, Whitehead R, Tang D, Whyatt RW: Impact of prenatal chlorpyrifos exposure on neurodevelopment in the first 3 years of life among inner-city children. Pediatrics. 2006, 118: e1845-59. 10.1542/peds.2006-0338.View ArticleGoogle Scholar
  23. Eskenazi B, Marks AR, Bradman A, Harley K, Barr DB, Johnson C, Morga N, Jewell NP: Organophosphate pesticide exposure and neurodevelopment in young Mexican-American children. Environ Health Perspect. 2007, 115: 792-8.View ArticleGoogle Scholar
  24. Chanda SM, Pope CN: Neurochemical and neurobehavioral effects of repeated gestational exposure to chlorpyrifos in maternal and developing rats. Pharmacol Biochem Behav. 1996, 53: 771-6. 10.1016/0091-3057(95)02105-1.View ArticleGoogle Scholar
  25. Laviola G, Adriani W, Gaudino C, Marino R, Keller F: Paradoxical effects of prenatal acetylcholinesterase blockade on neuro-behavioral development and drug-induced stereotypies in reeler mutant mice. Psychopharmacology (Berl). 2006, 187: 331-44. 10.1007/s00213-006-0426-z.View ArticleGoogle Scholar
  26. Clancy B, Finlay BL, Darlington RB, Anand KJ: Extrapolating brain development from experimental species to humans. Neurotoxicology. 2007, 28: 931-7. 10.1016/j.neuro.2007.01.014.View ArticleGoogle Scholar
  27. Bignami G: Economical test methods for developmental neurobehavioral toxicity. Environ Health Perspect. 1996, 104 (Suppl 2): 285-98. 10.2307/3432648.View ArticleGoogle Scholar
  28. Branchi I, Ricceri L: Transgenic and knock-out mouse pups: the growing need for behavioral analysis. Genes Brain Behav. 2002, 1: 135-41. 10.1034/j.1601-183X.2002.10301.x.View ArticleGoogle Scholar
  29. Fox WM: Reflex-ontogeny and behavioural development of the mouse. Anim Behav. 1965, 13: 234-41. 10.1016/0003-3472(65)90041-2.View ArticleGoogle Scholar
  30. Crawley JN: What's wrong with my mouse? Behavioral phenotyping of transgenic and knockout mice. 2000, Wiley-Liss, 32-39.Google Scholar
  31. Elwood RW, Keeling F: Temporal organization of ultrasonic vocalizations in infant mice. Dev Psychobiol. 1982, 15: 221-7. 10.1002/dev.420150306.View ArticleGoogle Scholar
  32. Ihnat R, White NR, Barfield RJ: Pup's broadband vocalizations and maternal behavior in the rat. Behav Processes. 1995, 33: 257-72. 10.1016/0376-6357(94)00028-F.View ArticleGoogle Scholar
  33. Noirot E, Pye D: Sound analysis of ultrasonic distress calls of mouse pups as a function of their age. Anim Behav. 1969, 17: 340-49. 10.1016/0003-3472(69)90020-7.View ArticleGoogle Scholar
  34. Hofer MA: Multiple regulators of ultrasonic vocalization in the infant rat. Psychoneuroendocrinology. 1996, 21: 203-17. 10.1016/0306-4530(95)00042-9.View ArticleGoogle Scholar
  35. Branchi I, Campolongo P, Alleva E: Scopolamine effects on ultrasonic vocalization emission and behavior in the neonatal mouse. Behav Brain Res. 2004, 151: 9-16. 10.1016/S0166-4328(03)00277-8.View ArticleGoogle Scholar
  36. Hofer MA: Early social relationships: a psychobiologist's view. Child Dev. 1987, 58: 633-47. 10.2307/1130203.View ArticleGoogle Scholar
  37. Hofer MA, Brunelli SA, Shair HN: Ultrasonic vocalization responses of rat pups to acute separation and contact comfort do not depend on maternal thermal cues. Dev Psychobiol. 1993, 26: 81-95. 10.1002/dev.420260202.View ArticleGoogle Scholar
  38. Hofer MA, Masmela JR, Brunelli SA, Shair HN: The ontogeny of maternal potentiation of the infant rats' isolation call. Dev Psychobiol. 1998, 33: 189-201. 10.1002/(SICI)1098-2302(199811)33:3<189::AID-DEV1>3.0.CO;2-N.View ArticleGoogle Scholar
  39. Tait S, Ricceri L, Venerosi A, Maranghi F, Mantovani A, Calamandrei G: Long-term effects on hypothalamic neuropeptides after developmental exposure to chlorpyrifos in mice. Environ Health Perspect. 2009, 117: 112-6.View ArticleGoogle Scholar
  40. Insel TR, Winslow JT: Central administration of oxytocin modulates the infant rat's response to social isolation. Eur J Pharmacol. 1991, 203: 149-52. 10.1016/0014-2999(91)90806-2.View ArticleGoogle Scholar
  41. Scattoni ML, McFarlane HG, Zhodzishsky V, Caldwell HK, Young WS, Ricceri L, Crawley JN: Reduced ultrasonic vocalizations in vasopressin 1b knockout mice. Behav Brain Res. 2008, 187: 371-8. 10.1016/j.bbr.2007.09.034.View ArticleGoogle Scholar
  42. Shapiro LE, Insel TR: Ontogeny of oxytocin receptors in rat forebrain: a quantitative study. Synapse. 1989, 4: 259-66. 10.1002/syn.890040312.View ArticleGoogle Scholar
  43. Winslow JT, Hearn EF, Ferguson J, Young LJ, Matzuk MM, Insel TR: Infant vocalization, adult aggression, and fear behavior of an oxytocin null mutant mouse. Horm Behav. 2000, 37: 145-55. 10.1006/hbeh.1999.1566.View ArticleGoogle Scholar
  44. Vandenbergh JG: Prenatal Hormone exposure and sexual variation. American Scientist. 2003, 91: 218-25.View ArticleGoogle Scholar
  45. Venerosi A, Valanzano A, Alleva E, Calamandrei G: Prenatal exposure to anti-HIV drugs: neurobehavioral effects of zidovudine (AZT) + lamivudine (3TC) treatment in mice. Teratology. 2001, 63: 26-37. 10.1002/1096-9926(200101)63:1<26::AID-TERA1005>3.0.CO;2-G.View ArticleGoogle Scholar
  46. Wilcox RG: New statistical procedures for the social sciences. 1987, Hillsdale NJ: Erlbaum, 187-189.Google Scholar
  47. Whyatt RM, Rauh V, Barr DB, Camann DE, Andrews HF, Garfinkel R, Hoepner LA, Diaz D, Dietrich J, Reyes A, Tang D, Kinney PL, Perera FP: Prenatal insecticide exposures and birth weight and length among an urban minority cohort. Environ Health Perspect. 2004, 112: 1125-32.View ArticleGoogle Scholar
  48. Maurissen JP, Hoberman AM, Garman RH, Hanley TR: Lack of selective developmental neurotoxicity in rat pups from dams treated by gavage with chlorpyrifos. Toxicol Sci. 2000, 57: 250-63. 10.1093/toxsci/57.2.250.View ArticleGoogle Scholar
  49. Blumberg MS, Alberts JR: Ultrasonic vocalizations by rat pups in the cold: an acoustic by-product of laryngeal braking?. Behav Neurosci. 1990, 104: 808-17. 10.1037/0735-7044.104.5.808.View ArticleGoogle Scholar
  50. Hofer MA: Unexplained infant crying: an evolutionary perspective. Acta Paediatr. 2002, 91: 491-6. 10.1080/080352502753711542.View ArticleGoogle Scholar
  51. LaGasse LL, Neal AR, Lester BM: Assessment of infant cry: acoustic cry analysis and parental perception. Ment Retard Dev Disabil Res Rev. 2005, 11: 83-93. 10.1002/mrdd.20050.View ArticleGoogle Scholar
  52. Newman JD: Neural circuits underlying crying and cry responding in mammals. Behav Brain Res. 2007, 182: 155-65. 10.1016/j.bbr.2007.02.011.View ArticleGoogle Scholar
  53. Elsner J, Suter D, Alder S: Microanalysis of ultrasound vocalizations of young rats: assessment of the behavioral teratogenicity of methylmercury. Neurotoxicol Teratol. 1990, 12: 7-14. 10.1016/0892-0362(90)90106-M.View ArticleGoogle Scholar
  54. De Marco M, Halpern R, Barros HM: Early behavioral effects of lead perinatal exposure in rat pups. Toxicology. 2005, 211: 49-58. 10.1016/j.tox.2005.02.007.View ArticleGoogle Scholar
  55. Rankin J, Manning A: Alterations to the pattern of ultrasonic calling after prenatal exposure to aluminium sulfate. Behav Neural Biol. 1993, 59: 136-42. 10.1016/0163-1047(93)90860-K.View ArticleGoogle Scholar
  56. Barron S, Gilbertson R: Neonatal ethanol exposure but not neonatal cocaine selectively reduces specific isolation-induced vocalization waveforms in rats. Behav Genet. 2005, 35: 93-102. 10.1007/s10519-004-0859-2.View ArticleGoogle Scholar
  57. Marino MD, Cronise K, Lugo JN, Kelly SJ: Ultrasonic vocalizations and maternal-infant interactions in a rat model of fetal alcohol syndrome. Dev Psychobiol. 2002, 41: 341-51. 10.1002/dev.10077.View ArticleGoogle Scholar
  58. Rautava L, Lempinen A, Ojala S, Parkkola R, Rikalainen H, Lapinleimu H, Haataja L, Lehtonen L: Acoustic quality of cry in very-low-birth-weight infants at the age of 1 1/2 years. Early Hum Dev. 2007, 83: 5-12. 10.1016/j.earlhumdev.2006.03.004.View ArticleGoogle Scholar
  59. Rothenberg SJ, Cansino S, Sepkoski C, Torres LM, Medina S, Schnaas L, Poblano A, Karchmer S: Prenatal and perinatal lead exposures alter acoustic cry parameters of neonate. Neurotoxicol Teratol. 1995, 17: 151-60. 10.1016/0892-0362(94)00066-M.View ArticleGoogle Scholar
  60. Arria AM, Derauf C, Lagasse LL, Grant P, Shah R, Smith L, Haning W, Huestis M, Strauss A, Della Grotta S, Liu J, Lester B: Methamphetamine and other substance use during pregnancy: preliminary estimates from the Infant Development, Environment, and Lifestyle (IDEAL) study. Matern Child Health J. 2006, 10: 293-302. 10.1007/s10995-005-0052-0.View ArticleGoogle Scholar
  61. Lester BM, Tronick EZ, LaGasse L, Seifer R, Bauer CR, Shankaran S, Bada HS, Wright LL, Smeriglio VL, Lu J, Finnegan LP, Maza PL: The maternal lifestyle study: Effects of substance exposure during pregnancy on neurodevelopmental outcome in 1-month-old infants. Pediatrics. 2002, 110: 1182-92. 10.1542/peds.110.6.1182.View ArticleGoogle Scholar
  62. Ricceri L: Behavioral patterns under cholinergic control during development: lessons learned from the selective immunotoxin 192 IgG saporin. Neurosci Biobehav Rev. 2003, 27: 377-84. 10.1016/S0149-7634(03)00068-X.View ArticleGoogle Scholar
  63. Levin ED: Nicotinic receptor subtypes and cognitive function. J Neurobiol. 2002, 53: 633-40. 10.1002/neu.10151.View ArticleGoogle Scholar
  64. Qiao D, Seidler FJ, Tate CA, Cousins MM, Slotkin TA: Fetal chlorpyrifos exposure: adverse effects on brain cell development and cholinergic biomarkers emerge postnatally and continue into adolescence and adulthood. Environ Health Perspect. 2003, 111: 536-44.View ArticleGoogle Scholar
  65. Joyce MP, Carden SE: The effects of 8-OH-DPAT and (+/-)-pindolol on isolation-induced ultrasonic vocalizations in 3-, 10-, and 14-day-old rats. Dev Psychobiol. 1999, 34: 109-17. 10.1002/(SICI)1098-2302(199903)34:2<109::AID-DEV4>3.0.CO;2-T.View ArticleGoogle Scholar
  66. Winslow JT, Shapiro L, Carter CS, Insel TR: Oxytocin and complex social behavior: species comparisons. Psychopharmacol Bull. 1993, 29: 409-14.Google Scholar
  67. Alberts JR: Huddling by rat pups: ontogeny of individual and group behavior. Dev Psychobiol. 2007, 49: 22-32. 10.1002/dev.20190.View ArticleGoogle Scholar


© Venerosi et al; licensee BioMed Central Ltd. 2009

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.