Figure 5From: Evaluation of two commercial and three home-made fixatives for the substitution of formalin: a formaldehyde–free laboratory is possible Immunostaining for WT-1. In Wilm's tumor (Cell-Block, PAGA and ZBF) staining is more intense in glomerular-like structures and in glomeruli of normal kidney (Z7 and ZBF). Some tubular cells are positive in the medulla only in PAGA-fixed tissue. [Manual antigen retrieval with microwave treatment in EDTA buffer pH 8.5.].Back to article page