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Table 4 Characteristics of enzymatic treatments used in indirect measures

From: A round robin approach to the analysis of bisphenol a (BPA) in human blood samples

  Laboratory # 2 Laboratory # 3 Laboratory # 4
Method source US CDC [46] US FDA/NCTR [76] Designed with high enzyme concentration
Enzyme source Helix pomatia Helix pomatia Type H-3 Helix pomatia Type H-1
Enzyme info Sigma-Aldrich Sigma-Aldrich Sigma Aldrich, G 0751
Number of units used 291.4 U 2 U 1000 U
Volume of sample 0.5 ml 0.25 ml 1 ml
pH of reaction 5 5.5 5
Length of reaction 12 h 2 h Overnight (~18 h)
Temperature of reaction 37°C 37°C 37°C