Table 2 Summary of the main effects induced by single chemicals administered at IC20, IC5 and IC20/100. In bracket, the lowest concentration eliciting a statistically significant modification of at least one of the measured DNT features is indicated (Phase 2)
3 days | BPA | CPF | Lead | Methyl-Hg | PCB138 | VA |
Synaptogenesis (SYP, PSD95) | ⇓ PSD95t (IC20) | ⇑ SYPt (IC20) | ⇓ PSD95n (IC20/100) | ⇔ | ⇑ PSD95n (IC20) | ⇔ |
Neurite outgrowth | ⇔⇑ n. neurites (IC5) | ⇓ ⇔ | ⇔ | ⇔ | ⇑ (IC20/100) | ⇑ n. neurites (not shown) (IC5) |
BDNF levels | ⇔ | ⇑ BDNFt (IC20) | ⇔ | ⇔ | ⇔ ⇑ ratio (IC20/100) | ⇔ |
14 days | BPA | CPF | Lead | Methyl-Hg | PCB138 | VA |
Synaptogenesis (SYP, PSD95) | ⇓ PSD95n (IC5) | ⇓ SYPn ⇑ PSD95t (IC5) | ⇑ SYPt ⇓ PSD95n (IC20/100) | ⇓ SYPn (IC5) | ⇔ | ⇔ |
Neurite outgrowth | ⇓ n. branch points (IC5) | ⇓ ⇔ | ⇔ | ⇓ n. branch points (IC20) | ⇔ | ⇔ |
BDNF levels | ⇔ ⇓ ratio (IC5) | ⇑ BDNFt (IC20) ⇓ ratio (IC20) | ⇔ | ⇓ ratio (IC20) | ⇑ (IC5) | ⇑ BDNFn and ratio (IC20) |