Fig. 4

The effects of DBP/MBP exposure on Peli2 expression. a, b Testicular sections were collected from pups 22 days after they were exposed in utero (GD12.5 - birth) to corn oil or DBP doses of 50, 250 or 500 mg/kg/day. The expression of Peli2 in mouse testicular tissues was carried out by immunohistochemistry. Arrows represent the expression of Peli2 in the testes of DBP-treated and control male pups. The ratio of positive cells was detected by ImageJ (n = 6). The expression of Peli2 in SCs after exposure to different concentrations of MBP for 24 h. c The mRNA levels of Peli2 were measured with quantitative PCR (q-PCR), and GAPDH was measured as a loading control. d, e Peli2 protein levels were measured by Western blotting. The densitometry data were quantified with ImageJ (n = 3). GAPDH was assessed as an internal control. The results are expressed as the means ± SEM. ** p < 0.01; * p < 0.05