Skip to main content

Table 6 Description and supporting evidence of the KEs included in postulated MoA for BPAF in male adult exposure

From: Assessment of the endocrine disrupting properties of bisphenol AF: a case study applying the European regulatory criteria and guidance

  Brief description of key event Supporting evidence
MIE 1 Androgen receptor inhibition (two parallel MIEs are suggested, current knowledge in endocrinology does not provide sufficient knowledge to conclude whether they are indeed parallel or whether one of them precedes the other) In vitro mechanistic:
Androgen receptor antagonist binding IDs: 75–76
Androgen receptor antagonist activity IDs: 19, 21–22, 43, 64, 78, 81, 87
In vivo mechanistic:
Prostate weight decrease (adult exposure) ID: 60
Epididymis weight decrease (adult exposure) ID: 88
Seminal vesicles weight decrease (adult exposure) ID: 60
MIE 2 Altered steroidogenesis In vitro mechanistic:
Steroidogenesis alteration IDs: 4–5, 87
In vivo mechanistic:
Steroidogenesis gene/protein expression alteration (adult exposure) IDs: 55, 58, 83
Testosterone level decrease in male (adult exposure) ID: 55
FSH level increase in male (adult exposure) ID: 55
LH level increase in male (adult exposure) ID: 55
KE1 Dysfunction of male reproductive organs EATS mediated:
Testis histopathology alteration (adult exposure) IDs: 60, 88
Prostate weight decrease (adult exposure) ID: 60
Epididymis weight decrease (adult exposure) ID: 88
Seminal vesicles weight decrease (adult exposure) ID: 60
Seminal vesicles histopathology alteration (adult exposure) ID: 88
AO Impaired male fertility Sensitive but not diagnostic of EATS:
Fertility decrease (adult exposure) ID: 88