Fig. 1

Chemotherapy dose–response in HEC-1 and Ishikawa cells. a Timeline of experiments. Dose-dependent reduction in survival fraction in (b) HEC-1 (orange) and (c) Ishikawa (purple) cells after exposure to 0 – 400 μM or 0 – 800 μM carboplatin for 48 h, respectively. Dose-dependent reduction in survival fraction in (d) HEC-1 and (e) Ishikawa cells after exposure to 0.1 – 50 μM cisplatin for 48 h. These results indicate chemotherapy sensitivity in both cell lines at baseline. n = 4 independent experiments in triplicate for HEC-1 carboplatin curves, n = 4 independent experiments in sextuplicate for Ishikawa carboplatin curves (n = 2 in sextuplicate for 1 μM and 800 μM groups), n = 3 independent experiments in sextuplicate for HEC-1 cisplatin curves, and n = 4 independent experiments in sextuplicate for Ishikawa cisplatin curves