Fig. 5

Perinatal exposure to DDTs decreased sympathetic innervation of BAT in adult female mice. a Representative images of BAT immunostained for tyrosine hydroxylase (TH, red), neuropeptide Y (NPY, green), and counterstaining with DAPI to identify cell nuclei. Fibers immunopositive for TH but not NPY innervate BAT; fibers immunopositive for both TH and NPY innervate blood vessels. Scale bar = 25 μm. b Quantification of adrenergic innervation as determined by % area TH+/NPY- per ROI, with n = 4–8 ROIs averaged per sample and n = 6–7 animals per treatment used to calculate the mean. Data represented as mean positive immunoreactivity per ROI normalized per 1000 DAPI+ nuclei ±SE. Statistical significance determined by one-way ANOVA with post hoc Tukey’s test. *p < 0.05 vs Veh. BAT, brown adipose tissue; Veh, vehicle (organic olive oil); DDT, dichlorodiphenyltrichloroethane; DDE, dichlorodiphenyldichloroethylene