Air pollution and anemia as risk factors for pneumonia in ecuadorian children: a retrospective cohort analysis
© Harris et al; licensee BioMed Central Ltd. 2011
Received: 16 May 2011
Accepted: 3 November 2011
Published: 3 November 2011
Ambient air pollution and malnutrition, particularly anemia, are risk factors for pneumonia, a leading cause of death in children under five. We simultaneously assessed these risk factors in Quito, Ecuador.
In 2005, we studied two socioeconomically similar neighborhoods in Quito: Lucha de los Pobres (LP) and Jaime Roldos (JR). LP had relatively high levels of air pollution (annual median PM2.5 = 20.4 μg/m3; NO2 = 29.5 μg/m3) compared to JR (annual median PM2.5 = 15.3 μg/m3; NO2 = 16.6 μg/m3). We enrolled 408 children from LP (more polluted) and 413 children from JR (less polluted). All subjects were aged 18-42 months. We obtained medical histories of prior physician visits and hospitalizations during the previous year, anthropometric nutrition data, hemoglobin levels, and hemoglobin oxygen saturation via oximetry.
In anemic children, higher pollution exposure was significantly associated with pneumonia hospitalization (OR = 6.82, 95%CI = 1.45-32.00; P = 0.015). In non-anemic children, no difference in hospitalizations by pollution exposure status was detected (OR = 1.04, NS). Children exposed to higher levels of air pollution had more pneumonia hospitalizations (OR = 3.68, 1.09-12.44; P = 0.036), total respiratory illness (OR = 2.93, 95% CI 1.92-4.47; P < 0.001), stunting (OR = 1.88, 1.36-2.60; P < 0.001) and anemia (OR = 1.45, 1.09-1.93; P = 0.013) compared to children exposed to lower levels of air pollution. Also, children exposed to higher levels of air pollution had significantly lower oxygen saturation (92.2% ± 2.6% vs. 95.8% ± 2.2%; P < 0.0001), consistent with air pollution related dyshemoglobinemia.
Ambient air pollution is associated with rates of hospitalization for pneumonia and with physician's consultations for acute respiratory infections. Anemia may interact with air pollution to increase pneumonia hospitalizations. If confirmed in larger studies, improving nutrition-related anemia, as well as decreasing the levels of air pollution in Quito, may reduce pneumonia incidence.
Pneumonia is the leading cause of childhood death [1, 2]. The pathogenesis of pneumonia includes environmental and host factors, such as air pollution and malnutrition . Air pollutants are respiratory irritants and increase susceptibility to acute respiratory infections (ARI) [3, 4]. Air pollution increases morbidity and mortality largely from respiratory diseases , and reducing ambient air pollution increases lifespan . Particulate air pollution (PM), especially particles < 10 μm (PM10) and < 2.5 μm (PM2.5) in aerodynamic diameter, and nitrogen oxides (NOx) are specifically linked to childhood ARI [7–9]. Outdoor (ambient) air pollution, and indoor pollution from biofuel use, have been identified as major pneumonia risk factors [2, 3].
Globally, many malnourished children are exposed to high levels of air pollution. Also, because malnourished children are known to be at increased risk for pneumonia [1, 2, 10, 11], a synergistic relationship between ambient air pollution and malnutrition has been postulated . Previous studies of pneumonia have examined either air pollution or malnutrition [e.g. [12–15]] but not both risk factors simultaneously. We explored the relationship between air pollution and malnutrition in Quito, Ecuador where both risk factors are prevalent. Our primary objective was to determine if interaction between malnutrition and air pollution was evident. To our knowledge this is the first study of possible interaction effects between ambient air pollution and malnutrition as risk factors for pneumonia in children.
This study was approved by the Tufts University Institutional Review Board and the Ethical Committee of the Corporación Ecuatoriana de Biotechnología (CEB).
Socioeconomic indicators in LP (higher air pollution) and JR (lower air pollution)
Superior and above (%)
Household electricity (%)b
Absence of poverty (%)
Working age population (%)
Employment by economic sector (persons)c
χ2 = 3.23 (2df)
Between February and May 2006 we enrolled 408 and 413 children aged 18-42 months in LP and JR, respectively, after using a neighborhood census to identify eligible children. For each child we obtained a medical history using a standardized, pre-tested questionnaire for parents asking if the child was hospitalized or received medical attention for a respiratory illness in the prior year, including diagnoses and treatments. Anthropometric measurements included height, weight, and mid-upper arm circumference (MUAC). Length (age < 24 months) or height (age ≥24 months) was measured (nearest millimeter) using a nondistensible plastic tape glued to a rigid board. Weight was measured (closest 0.1 kilogram) using Detecto® Health-o-meters balances and scales (Webb City, IA, USA). MUAC was measured using a nondistensible plastic tape (closest millimeter). Height and weight were converted into weight-for-age (WAZ) and height-for-age (HAZ) Z-scores using Centers for Disease Control (CDC) reference values in the EpiInfo software package . A sterile lancet was used to draw fingerstick blood into a microcuvette analyzer (Hemocue AB, Angelholm, Sweden). Pulse-oximetry blood hemoglobin oxygen saturation (SpO2) was assessed via an index finger sensor (opposite hand from fingerstick) after sensor readings stabilized. Anemic children were offered iron supplements. Altitude was measured at each house using a GPS receiver (Garmin, Olathe, KS, USA).
Statistical analysis was performed using EpiInfo (CDC, EpiInfo for Windows, version 3.4.1) and SPSS for Windows (SPSS Incorporated, SPSS Release 14.0). Demographic and illness data were compared using Pearson χ2 analysis and Fisher's exact test. Logistic regression models were used for the multivariate analyses of both outcomes: hospitalization due to pneumonia and outpatient consultations due to acute respiratory illness (ARI). Associations were assessed by calculation of the odds ratio (OR) with 95% confidence intervals (CI). Following international conventions, children with height for age (HAZ) z-scores ≤ 2 standard deviations below the mean age and gender adjusted means were termed stunted. Anemia was defined as an adjusted blood hemoglobin < 11.0 g/dL after subtracting 1.9 g/dL to adjust for altitude . In order to quantitatively check our assumption of significant air pollution differences by neighborhood, median air pollution levels were tested using the Mann-Whitney test.
Demographic, nutritional, and environmental information for children exposed to higher (LP), and lower (JR), air pollution.
N = 401
N = 397
Males (number, %)
Presence of stuntinga
Adjusted hemoglobin (g/dL)b
Presence of anemiac
Mean household altitude (m)
Hospitalizations due to pneumonia, physician consultations for ARI, and air pollution exposure
All 22 hospitalizations were for pneumonia. Of the hospitalized children, 7 resided in the lower air pollution exposure neighborhood while 15 resided in the higher air pollution exposure neighborhood. Parents were able to recall a specific diagnosis for 97% of physician consultations for all ailments in 2005 and this percentage did not vary between neighborhoods. The most common outpatient diagnoses were asthma, bronchitis, cough, influenza, pharyngitis, pneumonia, sinusitis, and tonsillitis. A total of 172 of 397 children (43%) with lower air pollution exposure required one or more consultations versus 224 of 401children (56%) with higher air pollution exposure.
Hospitalization for pneumonia: Logistic regression analysis controlling for cofactors.
(N = 792)
(N = 331)
(N = 461)
Air pollution exposure*
Medical consultations for respiratory illness: Logistic regression analysis controlling for cofactors.
(N = 792)
(N = 331)
(N = 461)
Air pollution exposure*
There were no significant differences in age, gender, or nutritional parameters (HAZ, WAZ, MUAC, hemoglobin) between previously ill and healthy children. However, SpO2 was significantly lower in previously ill children compared to healthy children (93.6 ± 3.0% vs. 94.5 ± 3.0%; P < 0.001). In chi-square and regression analyses, neighborhood SES factors were not associated with any nutritional, environmental, or illness characteristics or outcomes.
Modification of the effect of air pollution exposure by anemia
Fifteen of the 22 hospitalized children were anemic. The presence of anemia increased the OR for pneumonia hospitalization in children with high air pollution exposure from 3.68 to 6.82 (Table 3). In the absence of anemia, higher air pollution exposure was not predictive of pneumonia hospitalization (OR 1.04; 95% CI 0.08-13.93). In contrast, anemia status did not change the influence of air pollution exposure on ARI events that required consultation with a physician (Table 4).
Our most important result is that anemic children exposed to relatively high levels of ambient air pollution are at increased risk of pneumonia hospitalization compared to children exposed to lower levels of air pollution. In addition, we found that children (both anemic and non-anemic) who were exposed to higher levels of air pollution were significantly more likely to have received a physician consultation for acute respiratory illness compared to children exposed to lower levels of air pollution. We also found evidence that anemia and higher air pollution exposure may interact to increase the odds of hospitalization in children with pneumonia. The strongest evidence of the potential interaction effect is that anemia increased the OR for the association between higher air pollution exposure and pneumonia hospitalization from OR (3.68, 95% CI 1.09-12.44; P = 0.036) for all children to OR 6.82 (95% CI 1.45-32.00; P = 0.015) for anemic children. In non-anemic children, the OR for hospitalization with pneumonia in children exposed to higher levels of air pollution dropped from 3.68 (noted above) to 1.04 (95% CI 0.08-13.9; P = 0.98). These results suggest a possible interaction between anemia and ambient air pollution exposure as risk factors. This result is important as these risk factors have not been examined concurrently before, however, our study did not have the statistical power to formally assess the potential interaction between pneumonia hospitalization and anemia status. Larger studies specifically designed to examine this finding are required.
The central pathophysiological deficit in pneumonia is poor tissue oxygenation . Anemia and low hemoglobin oxygen saturation independently decrease oxygen delivery. Hypoxia increases mortality during pneumonia two to five-fold [23, 24]. The association between anemia and pneumonia has been recognized since at least 1925 . Anemic children have less oxygen carrying capacity-and suffer greater hypoxia during pneumonia-than normal children . Oxygen saturation was significantly lower (P < 0.001) in children with high air pollution exposure. It is biologically plausible that anemia, and the lower hemoglobin oxygen saturation of higher air pollution exposure children, could additively or multiplicatively decrease tissue oxygenation during pneumonia, especially at high altitude, resulting in more severe disease and more hospitalizations.
Reasons for differential oxygen saturations in normal populations are limited to only two possibilities: either marked differences in altitude, which was not the case in our study (127 m difference), or dyshemoglobinemia . Carbon monoxide (CO) and NOx (particularly NO), produced by vehicular engine combustion, reduce oxygen saturation through formation of the dyshemoglobins carboxyhemoglobin and methemoglobin . Vehicular engine combustion is the major source of air pollution in Quito [12, 16, 17].
Previously, Estrella et al.  documented elevated carboxyhemoglobin levels in primary school children in Quito. Carboxyhemoglobin levels were 2.52% ± 1.12 in a northern Quito school adjacent to our lower pollution neighborhood, and 5.09% ± 1.7 (P < 0.05) in a central city school, adjacent to our higher air pollution neighborhood. Carboxyhemoglobin levels above 2.5% are abnormal; 43% of children living in northern Quito and 92% of the children living in central Quito exceeded this threshold . Rare causes of dyshemoglobinemia (anesthetic or dapsone use, high-nitrate water ingestion), are unlikely alternative explanations since few children in this study would have undergone anesthesia or dapsone treatment, and all children shared a low-nitrate municipal water supply. Therefore, the most likely explanation for the marked SpO2 difference between the higher and lower air pollution exposure children is their differential exposure to common, combustion-related, air pollutants that bind to hemoglobin.
One potential limitation of our O2 saturation data was that we used non-invasive pulse oximetry rather than directly measuring carboxyhemoglobin and methemoglobin levels, as the latter would have required venous blood sampling, potentially decreasing study participation. However, oximetry underestimates carboxyhemoglobin-related oxygen saturation deficits [26, 27], suggesting the deficits we found are conservatively estimated.
This study supports interaction between ambient air pollution levels, anemia, and hospitalization for pneumonia because (1) the OR increased in the setting of both risk factors, and (2) a biologically plausible pathophysiological mechanism for synergy can be identified. However, our study was designed for a straightforward comparison and sample size was too small to conclusively test for significant interaction in either tabular analysis using the Breslow-Day approach or by using an interaction term in the logistic regression model. Mauderly and Samet (2009) noted the difficulty of establishing synergism in air pollution studies because sample sizes of people with impaired health are generally too low for rigorous statistical testing . This study, however, is informative for the design of larger studies looking specifically for evidence of this interaction.
The study children resided in impoverished neighborhoods (Table 1) which were carefully chosen to minimize differential nutritional status secondary to family socioeconomic status. We hypothesize that the incrementally worse nutritional status (HAZ, WAZ, and anemia) of the higher exposure children (Table 2) was due to their incrementally higher incidence of respiratory infections related to higher air pollution (Tables 3 and 4). Higher rates of infection are known to worsen nutritional status, including anemia, in children [1, 2, 10, 11, 29].
It could be argued that the increased pneumonia hospitalization of the higher air pollution exposure children was due to their greater malnutrition, and not their air pollution exposure. However, in the entire study population, children with and without a history of respiratory illness did not differ by age, gender, or any nutritional parameters (HAZ, WAZ, MUAC, hemoglobin). The only significant difference between ill and healthy children was the lower oxygen saturation level seen in children with a pneumonia hospitalization or physician consultation (93.6 ± 3.0% vs. 94.5 ± 3.0%; P < 0.001). We believe this difference in SpO2 represents air pollution-related dyshemoglobinemia.
Data from a retrospective study must be interpreted cautiously. First, although the site populations did not differ by SES metrics, we did not collect household SES data that potentially could have provided additional insight into the role(s) of various socioeconomic factors. Second, families had to recall health information regarding their children over the previous 12 months. Although we inquired about events that were likely to be remembered (indeed, specific diagnoses were recalled for all (100%) hospitalizations and 495 of 512 (97%) physician consultations), we could not independently verify that all recalled diagnoses were accurate. Third, we did not correct for some potential confounding variables such as biofuel smoke in indoor air or cigarette smoking, however, ~ 98% of households in both sites used gas and not biofuels (Table 1), and therefore it is unlikely that biofuel smoke introduced a major source of bias. Also, in a tobacco-use survey (Harris et al, unpublished data), 30% of adults in LP and JR stated that they smoked cigarettes but 96% of users smoked ≤5 cigarettes per day because of cost. Furthermore, Estrella et al  found no difference in carboxyhemoglobinemia in children from households with and without tobacco use. These factors suggest that smoking was not a major source of bias in our study. We used data for 2005 from CORPAIRE, which monitors ambient air pollution using accepted methods [17, 30] and we assumed that living in a more polluted neighborhood was linked to higher personal exposure to ambient air pollution, a widely accepted assumption . Lastly, it is possible that unknown confounding factors we did not measure accounted for the higher rates of pneumonia, rather than the combination of air pollution and anemia.
In summary, we found evidence of possible synergy between a common manifestation of malnutrition-anemia-and ambient air pollution as risk factors for pneumonia hospitalization in a high-altitude setting. Their effects on oxygen delivery could plausibly mediate this association. We suspect air pollution-related dyshemoglobinemia reduces oxygen saturation, while anemia decreases oxygen carrying capacity. Our study suggests that both improving nutrition, and controlling air pollution, could most rapidly decrease hospitalizations for pneumonia, the leading cause of death in children. Further study is needed to confirm our finding of synergy between air pollution and anemia.
This work was supported by US National Institutes of Health grants R01 HD38327, R21TW006537, and F30ES012550. No author has a financial interest in the results of this study, and the views expressed are those of the authors and not the funding agency.
We thank the staff of the Corporación Ecuatoriana de Biotechnología; the Corporación Para el Mejoramiento del Aire de Quito (for geocoded maps); and Drs. Doug Brugge and Patricia Hibberd for insightful comments.
- Mulholland K: Childhood pneumonia mortality-a permanent global emergency. Lancet. 2007, 370: 285-289. 10.1016/S0140-6736(07)61130-1.View ArticleGoogle Scholar
- Rudan I, Boschi-Pinto C, Biloglav Z, Mulholland K, Campbell H: Epidemiology and etiology of childhood pneumonia. Bull World Health Organ. 2008, 86: 408-416. 10.2471/BLT.07.048769.View ArticleGoogle Scholar
- Bruce N, Perez-Padilla R, Albalak R: Indoor air pollution in developing countries: a major environmental and public health challenge. Bull World Health Organ. 2000, 78: 1078-92.Google Scholar
- Chuang K-J, Chan C-C, Su , Ta-Chen , Lee C-T, Tang C-S: The effect of urban air pollution on inflammation, oxidative stress, coagulation, and autonomic dysfunction in young adults. Am J Respir Crit Care Med. 2007, 176: 370-376. 10.1164/rccm.200611-1627OC.View ArticleGoogle Scholar
- Samet JM, Dominici F, Curriero FC, Coursac I, Zeger SL: Fine particulate air pollution and mortality in 20 U.S. cities, 1987-1994. N Engl J Med. 2000, 343: 1742-9. 10.1056/NEJM200012143432401.View ArticleGoogle Scholar
- Pope CA, Ezzati M, Dockery DW: Fine-particulate air pollution and life expectancy in the United States. N Engl J Med. 2009, 360: 376-386. 10.1056/NEJMsa0805646.View ArticleGoogle Scholar
- Glinianaia SV, Rankin J, Bell R, Pless-Mulloli T, Howel D: Does particulate air pollution contribute to infant death? A systematic review. Environ Health Perspect. 2004, 112: 1365-1371. 10.1289/ehp.6857.View ArticleGoogle Scholar
- Kraft M, Eikmann T, Kappos A, Kunzli N, Rapp R, Schneider K, Seitz H, Voss J-U, Wichmann HE: The German view: effects of nitrogen dioxide on human health--derivation of health-related short-term and long-term values. Int J Hyg Environ Health. 2005, 208: 305-318. 10.1016/j.ijheh.2005.04.002.View ArticleGoogle Scholar
- Romieu I, Samet JM, Smith KR, Bruce N: Outdoor air pollution and acute respiratory infections among children in developing countries. J Occup Environ Med. 2002, 44: 640-649. 10.1097/00043764-200207000-00010.View ArticleGoogle Scholar
- Bryce J, Boschi-Pinto C, Shibuya K, Black RE: WHO estimates of the causes of death in children. Lancet. 2005, 365: 1147-1152. 10.1016/S0140-6736(05)71877-8.View ArticleGoogle Scholar
- Rice AL, Sacco L, Hyder A, Black RE: Malnutrition as an underlying cause of childhood deaths associated with infectious diseases in developing countries. Bull World Health Organ. 2000, 78: 1207-1221.Google Scholar
- Estrella B, Estrella R, Oviedo J, Narvaez X, Reyes MT, Gutierrez M, Naumova EN: Acute respiratory diseases and carboxyhemoglobin status in school children of Quito, Ecuador. Environ Health Perspect. 2005, 113: 607-611. 10.1289/ehp.7494.View ArticleGoogle Scholar
- Mishra V, Retherford RD: Does biofuel smoke contribute to anaemia and stunting in early childhood?. Int J Epidemiol. 2007, 36: 117-129.View ArticleGoogle Scholar
- Naumova EN, Yepes H, Griffiths JK, Sempertegui F, Khurana G, Jagai JS, Jativa E, Estrella B: Emergency room visits for respiratory conditions in children increased after Guagua Pichincha volcanic eruptions in April 2000 in Quito, Ecuador observational study: time series analysis. Environ Health. 2007, 6: 21-10.1186/1476-069X-6-21.View ArticleGoogle Scholar
- Hamer DH, Sempertegui F, Estrella B, Tucker KL, Egas J, Dallal GE, Selhub J, Griffiths JK, Meydani SN: Micronutrient deficiencies are associated with impaired immune response and higher burden of respiratory infections in elderly Ecuadorians. J Nutr. 2009, 139: 113-119.View ArticleGoogle Scholar
- Brachtl MV, Durant JL, Perez CP, Oviedo J, Sempertegui F, Naumova EN, Griffiths JK: Spatial and temporal variations and mobile source emissions of polycyclic aromatic hydrocarbons in Quito, Ecuador. Environ Pollut. 2009, 157: 528-536. 10.1016/j.envpol.2008.09.041.View ArticleGoogle Scholar
- CORPAIRE: La calidad del aire en Quito: Informe del primer ano de operación de la REMMAQ. June 2003-May 2004. Annual report. Quito, Ecuador. 2004Google Scholar
- Sempertegui F, Estrella B, Camaniero V, Betancourt V, Izurieta R, Ortiz W, Fiallo E, Troya S, Rodriguez A, Griffiths JK: The beneficial effects of weekly low-dose vitamin A supplementation on acute lower respiratory infections and diarrhea in Ecuadorian children. Pediatrics. 1999, 104: e1-10.1542/peds.104.1.e1.View ArticleGoogle Scholar
- Sempertegui F, Estrella B, Correa E, Aguirre L, Saa B, Torres M, Navarrete F, Carrion AC, Rodriguez J, Griffiths JK: Effects of short-term zinc supplementation on cellular immunity, respiratory symptoms, and growth of malnourished Equadorian children. Eur J Clin Nutr. 1996, 50: 42-46.Google Scholar
- Centers for Disease Control and Prevention: EpiInfo for Windows: Version 3.4.1. 2007Google Scholar
- Dirren H, Logman MH, Barclay DV, Freire WB: Altitude correction for hemoglobin. Eur J Clin Nutr. 1994, 48: 625-32.Google Scholar
- Brockway J, Hay WW: Prediction of arterial partial pressure of oxygen with pulse oxygen saturation measurements. J Pediatr. 1998, 133: 63-66. 10.1016/S0022-3476(98)70179-9.View ArticleGoogle Scholar
- Cantin AM: Childhood pneumonia and oxygen treatment. Lancet. 2008, 373: 1278-1280.View ArticleGoogle Scholar
- Lozano JM: Epidemiology of hypoxaemia in children with acute lower respiratory infection. Int J Tuberc Lung Dis. 2001, 5: 496-504.Google Scholar
- Bass MH: The association of anemia and pneumonia in infants. Am J Dis Child. 1925, 29: 318-328.View ArticleGoogle Scholar
- Nagel RE, Jaffe ER: CO-, NO-, met-, and sulf-hemoglobinemias: The dyshemoglobins. Disorders of hemoglobin: Genetics, pathophysiology, and clinical management. Edited by: Steinberg MH, Forget BG, Higgs RD, Nagel RL. 2001, New York: Cambridge University Press, 1214-1233.Google Scholar
- Barker SJ, Tremper KK: The effect of carbon monoxide inhalation on pulse oximetry and transcutaneous PO2. Anesthesiology. 1987, 66: 677-679. 10.1097/00000542-198705000-00014.View ArticleGoogle Scholar
- Mauderly JL, Samet JM: Is there evidence for synergy among air pollutants in causing health effects?. Environ Health Perspect. 2009, 117: 1-6.View ArticleGoogle Scholar
- Lutter CK: Iron deficiency in young children in low-income countries and new approaches for its prevention. J Nutr. 2008, 138: 2523-2528. 10.3945/jn.108.095406.View ArticleGoogle Scholar
- CORPAIRE: Inventario de emisiones del distrito metropolitano de Quito. 2003, CORPAIRE 2006. Quito, EcuadorGoogle Scholar
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